2023 Fiscal Year Final Research Report
Genetic profiling of FAP-positive cancer-associated fibroblasts and exploratory studies for personalized therapy
| Project/Area Number |
22K20825
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0901:Oncology and related fields
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | オルガノイド / 食道癌 / 癌関連線維芽細胞 |
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| Outline of Final Research Achievements |
Clinical specimens of esophageal squamous cell carcinoma and normal esophageal epithelium were collected to generate an organoid library. Cultured cancer-associated fibroblasts were also established from esophageal cancer area. The success rates were 41% for cancer organoids, 78% for normal esophageal epithelial organoids, and 74% for cancer-associated fibroblasts, which were comparable to previous reports.
Phenotypes of organoids were different in each biopsy tissue, and expressed proteins were also different in each organoid, which reflects the characteristics of the biopsied tissue. The response of organoids to drugs may be predictive of drug resistance in biopsied patients.
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| Free Research Field |
消化器癌
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| Academic Significance and Societal Importance of the Research Achievements |
生検組織、つまり患者ごとに異なるオルガノイドおよび線維芽細胞を用いて、その特徴・各種薬剤に対する反応を解析することで食道癌をプロファイリングすることが可能となる．これにより同じ食道癌でも患者ごとに異なる最適な治療を行うための個別化治療の実現化につながる．早期再発症例や各種治療抵抗性症例に対しても既存の治療方法以外の選択肢を見出すことができるなら難治性食道がん患者に与える恩恵は計り知れない．
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