Establishment of ex vivo culture system and novel drug sensitivity test using EUS-FNA specimens from pancreatic cancer

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20827
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0901:Oncology and related fields
• Research Institution: Nagasaki University
• Principal Investigator: Kosuke Takahashi 長崎大学, 病院(医学系), 助手 (60957439)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: オルガノイド

Outline of Final Research Achievements

Tissue samples from 20 cases of pancreatic cancer that underwent EUS-FNA were analyzed for sample area measurement and tumor cellularity. The median tissue section area was 4.79 mm2 overall, with the breakdown by needle size being 9.06 mm2 for 19G, 4.85 mm2 for 22G, and 1.51 mm2 for 25G. Additionally, tumor cellularity was 28.3% (range 11-74%). It was found that EUS-FNB samples, particularly with larger needle diameters, allowed for the collection of more tissue. Furthermore, an attempt was made to create human-derived organoids using resected specimens and EUS-FNA samples for a novel drug sensitivity test. Ultimately, long-term maintenance and culture with three-dimensional cultivation methods were successfully achieved.

Free Research Field

消化器内科

Academic Significance and Societal Importance of the Research Achievements

今回の研究成果により現在使用されている穿刺針による採取組織量が明確となり、得られたEUS-FNA検体を用いてオルガノイド培養を安定して行えることが明らかとなった。今後の研究継続により新しい DST システムを確立していくことができれば、膵癌の実臨床における分子病理学的なデータと統合することによって、膵癌の個別化医療の確立に一役を担える事だけでなく、他のがんにも有益な結果をもたらす可能性がある。