Quantitative analysis of mast cells in allergic diseases

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20883
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0902:General internal medicine and related fields
• Research Institution: University of Yamanashi
• Principal Investigator: Vtran Nguyen 山梨大学, 大学院総合研究部, 特任助教 (30961346)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: マスト細胞 / アレルギー / 透明化技術

Outline of Final Research Achievements

Visualization of mast cells using tissue-clearing technology21 in Mcpt5-Cre TdTomatofl/fl mice enable us to better understand spatial and temporal distribution of mast cells in various organs such as skin and will be useful to evaluate therapeutic effects of allergy drugs on mast cells in vivo. In this study, we found the reduction of skin mast cells spatially in 3D after 7-week treatment with 100 mg/kg of MOD000001, a selective KIT inhibitor by using the fluorescence microscope-based findings in Mcpt5-Cre TdTomatofl/fl mice. These findings suggest that visualization of mast cells by this technology will help us understand the mechanisms behind mast cell-targeted therapy in allergic diseases.

Free Research Field

アレルギー学

Academic Significance and Societal Importance of the Research Achievements

本研究成果を元にして、マスト細胞の空間的時間的な状態を生体内で可視化することによって、アレルギー疾患のみならず自己免疫疾患や癌、線維化疾患などにおけるマスト細胞の役割が明らかになる。さらにマスト細胞を標的とした抗アレルギー作用を持つ薬剤の開発既存の抗アレルギー薬の作用を再評価することなどが期待できる。