Mechanism of action in the treatment of autologous nerve grafts with transvenous systemic administration of ADSCs.

2023 Fiscal Year Final Research Report

• Project/Area Number: 22K20969
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0906:Surgery related to the biological and sensory functions and related fields
• Research Institution: Kanazawa University
• Principal Investigator: Honda Soichiro 金沢大学, 附属病院, 医員 (10965362)
• Project Period (FY): 2022-08-31 – 2024-03-31
• Keywords: 脂肪由来幹細胞 / 自家神経移植術 / 全身投与

Outline of Final Research Achievements

We made Autologous nerve graft model rats and cell suspensions of ADSCs. We administered ADSCs via the tail vein of the model rats and compared with the control group.
In a study on the effect of systemically administered ADSCs on autologous nerve grafts, an increase in muscle wet weight of the tibialis anterior muscle and a shortening of the weekend latency of the compound muscle action potential were observed in the model treated with ADSCs, suggesting that nerve regeneration was accelerated. A study examining the distribution of systemically administered ADSCs found a small distribution of ADSCs around autologous nerve grafts. For the mechanism of action of systemically administered ADSCs, RNA sequencing was performed in ADSCs-treated and control groups. The results are currently being evaluated and will be reported in the future.

Free Research Field

整形外科

Academic Significance and Societal Importance of the Research Achievements

本研究によって、脂肪由来幹細胞を経静脈的に投与した場合においても、神経再生を促進する効果があることが判明した。全身投与した脂肪由来神座細胞は、その大部分が神経再生を促進する効果を持つ液性因子などを分泌し、それらを介して神経再生の促進に寄与していると考えている。本研究の社会的意義は、自家神経移植時におけるADSCsの低侵襲かつ簡便な投与方法の確立に貢献することである。