2023 Fiscal Year Final Research Report
Investigation of periodontal pathogens- intestinal epithelial interactions using 3D intestinal organoids
Project/Area Number |
22K21057
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0907:Oral science and related fields
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Research Institution | Niigata University |
Principal Investigator |
Mineo Shuhei 新潟大学, 医歯学総合病院, 医員 (20962814)
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Project Period (FY) |
2022-08-31 – 2024-03-31
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Keywords | ペリオドンタルメディスン / 歯周病原細菌 / オルガノイド |
Outline of Final Research Achievements |
In periodontal research, swallowed periodontopathogenic bacteria has been reported to have adverse systemic effects via the gut dysbiosis. Our laboratory has previously demonstrated that swallowed periodontopathogenic bacteria is involved in disrupting the intestinal epithelial barrier and inducing inflammation locally, but the detailed mechanisms remain unclear. In this study, we aimed to establish a bacterial infection model using intestinal organoids to examine the direct effects of oral- intestinal epithelial interactions. First, stem cells were isolated from mouse intestinal tracts, and organoid culture techniques were established. Subsequently, we attempted bacterial infection using an injector, but due to technical difficulties,we considered an alternative technique. As a result, we established a technique to change cell polarity and confirmed its usefulness as a model for bacterial infection.
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Free Research Field |
口腔科学
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Academic Significance and Societal Importance of the Research Achievements |
生体内環境を模倣可能な3次元腸管オルガノイドを用いて歯周病原細菌-腸管上皮相互作用を明らかにすることで,口腔-腸管連関におけるペリオドンタルメディスン病因論の理解を深めるという学術的意義を有する.また社会的意義として,本研究成果から動物モデルに替わる新たな解析技術として一般化することで,動物実験の代替・削減につながり,動物愛護の観点からの社会的貢献が期待される.
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