2023 Fiscal Year Final Research Report
Analysis of NO-Related Proteins in Human Autopsy Samples for Insights into Ischemic Heart Disease
| Project/Area Number |
22K21083
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0908:Society medicine, nursing, and related fields
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| Research Institution | Kobe University |
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Principal Investigator |
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | NO関連タンパク質 / 虚血性心疾患 / iNOS / NOS2 / ヒト剖検試料 |
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| Outline of Final Research Achievements |
In this study, coronary arteries and myocardium obtained from autopsies were analyzed to investigate the association between ischemic heart disease and nitric oxide-related proteins.
The concentrations of inducible nitric oxide synthase (iNOS/NOS2), endothelial nitric oxide synthase (eNOS/NOS3), and soluble guanylate cyclase (sGC) were quantified using ELISA in the homogenates of each sample and normalized to the total protein content. Statistical analysis comparing samples from hearts with and without coronary arteriosclerosis revealed a higher tendency of iNOS expression in myocardial samples with arteriosclerosis.
This study focused on a binary classification of the presence of coronary arteriosclerosis. Future research planned to expand the sample size and explore variations in iNOS expression relative to the severity of coronary arteriosclerosis.
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| Free Research Field |
Foresic Pathology
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、剖検で採取された心臓組織のホモジネート中に存在する可溶性グアニル酸シクラーゼ(sGC)・誘導型一酸化窒素合成酵素(iNOS)、内皮型一酸化窒素合成酵素(eNOS)の3つの一酸化窒素(NO)関連タンパク質について分析したところ、総タンパクで補正した心筋ホモジネート中のiNOS存在比は、冠動脈硬化のある事例で有意に高かった。iNOSはマクロファージなどによって誘導されるNO合成酵素であり、冠動脈硬化の発生にも大きく関わっているタンパク質である。そのため、超早期の虚血性心疾患の死後診断にも有用である可能性が示唆される。
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