2022 Fiscal Year Research-status Report
In vitro study of the association between ALDH2 rs671 polymorphism and Acral Lentiginous Melanoma
| Project/Area Number |
22K21117
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Keywords | Melanoma / Melanocytes / Melanin / Keratinocytes / Ethanol / Acetaldehyde |
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| Outline of Annual Research Achievements |
Malignant melanoma is a highly aggressive cancer that primarily originates from melanocytes in the epidermis. Over the past few decades, its incidence has been steadily increasing. Acral Lentiginous Melanoma (ALM) is a subtype of melanoma that develops on the palms and soles and is associated with a particularly poor prognosis. This project aims to explore whether Acral Lentiginous Melanoma is linked to acetaldehyde, a potentially relevant factor. To address this question, we first conducted an evaluation of melanin production using different reagents, including Acetaldehyde (ranging from 10 to 10^4 μM), Ethanol (0.01%), and α-MSH (at concentrations of 10^3 and 10^4 μM). The evaluation was performed at 6 and 24-hour time points, comparing the results to a control group. Intracellular melanin content was quantified using colorimetric methods. The initial findings indicate that acetaldehyde and α-MSH, at specific concentrations and time points, have the potential to influence intracellular melanin production in melanoma cells. However, the impact of ethanol seems to be negligible based on the tested concentrations. Further analysis and additional experiments are necessary to fully comprehend the underlying mechanisms and validate these preliminary findings.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
It seems it is according to the schedule
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| Strategy for Future Research Activity |
In the next phase of the project, we plan to conduct further steps, including evaluating keratinocyte melanin production in isolation, exploring melanin production in combination with melanocytes, and conducting molecular studies.
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| Causes of Carryover |
To use the remaining found for further experimental reagents and experimental equipment such as Co2 Cylinder, pipetman, and pipet.
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