2022 Fiscal Year Annual Research Report
Assessment of PilVax nasal vaccine for preventing influenza virus infection
| Project/Area Number |
22F20801
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| Allocation Type | Single-year Grants |
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| Research Institution | Chiba University |
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| Host Researcher |
藤橋 浩太郎 千葉大学, 医学部附属病院, 特任教授 (50820354)
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| Foreign Research Fellow |
TSAI JIA-YUN 千葉大学, 医科学研究所, 外国人特別研究員
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| Project Period (FY) |
2022-07-27 – 2024-03-31
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| Keywords | Nasal Vaccine / Influenza virus / IgA / Protection |
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| Outline of Annual Research Achievements |
Age-matched female BALB/c mice were immunized with of PilVax vaccines (1 X 10^8 CFU, in a volume of 10 ul) presenting two tandem copies (M2e-M2e-PilVax) of the M2e peptide. The vaccines were administered on 3 consecutive days with additional 3 boosters given 2 weeks apart, for a total of 12 doses. One week after the final immunization (day 51 or 49), serum, bronchoalveolar lavage (BAL) nasal washes (NWs) and saliva were collected and subjected to M2e-specific ELISAs. Significantly increased levels of M2e-specific serum IgG and mucosal IgA antibody responses were noted.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Age-matched female BALB/c mice were immunized with of PilVax vaccines (1 X 10^8 CFU, in a volume of 10 ul) presenting two tandem copies (M2e-M2e-PilVax) of the M2e peptide. The vaccines were administered on 3 consecutive days with additional 3 boosters given 2 weeks apart, for a total of 12 doses. One week after the final immunization (day 51 or 49), serum, bronchoalveolar lavage (BAL) nasal washes (NWs) and saliva were collected and subjected to M2e-specific ELISAs. Significantly increased levels of M2e-specific serum IgG and mucosal IgA antibody responses were noted.
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| Strategy for Future Research Activity |
Tissue samples will be isolated from nasal mucosa and lungs and subjected to immunohistochemical analyses by using confocal laser scanning microscopy in order to characterize the distribution of dendritic cells, T and B cells. Furthermore, we will test the functional properties of the M2e-specific antibodies by challenging vaccinated mice with influenza virus strains (H1N1, e.g., A/California and/or H3N2, e.g., A/Guizhou) to confirm protective immunity.
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