Elucidating the Mechanisms of Palatal Development by Combining Molecular Biology and Information Biology

2023 Fiscal Year Annual Research Report

• Project/Area Number: 22KF0265
• Allocation Type: Multi-year Fund
• Research Institution: Okayama University
• Principal Investigator: 大橋 俊孝 岡山大学, 医歯薬学域, 教授 (50194262)
• Co-Investigator(Kenkyū-buntansha): WANG ZIYI 岡山大学, 医歯薬学域, 外国人特別研究員
• Project Period (FY): 2023-03-08 – 2024-03-31
• Keywords: 口蓋発生 / single cell RNA-seq

Outline of Annual Research Achievements

王博士は公開されている大規模なゲノムワイド関連解析データを活用した口蓋裂の発生に関わる一塩基多型(SNP)解析およびマウス口蓋組織の期間培養実験を行い、メカニカルストレスとWntシグナル経路が口蓋の発生において重要な役割を担っている事実をつかんだ。さらに詳細なメカニズムを明らかにするために、1細胞遺伝子解析や空間的遺伝子解析など最新の技術を取り入れ、口唇・口蓋を含めた顎顔面の発生メカニズムを研究してきた。
本年度の研究で、in vivoの口蓋シェルフサンプルの口蓋融合領域で高発現しているフィラメントA（Flna）遺伝子を同定した。さらに、Flnaを標的とするsiRNAを用いたHaCaT細胞を用いたin vitro実験の結果から、FlnaがRho、Wnt、Smadシグナル伝達経路を制御している可能性が示唆された。口蓋裂のリスクを低減できる可能性のある口蓋融合の重要なメカニズムを明らかにした。

Research Products

• [Journal Article] Antitumor activity of α‐pinene in T‐cell tumors (2024)
  • Author(s): Masaya Abe; Noboru Asada; Maiko Kimura; Chie Fukui; Daisuke Yamada; Ziyi Wang; Masayuki Miyake; Takeshi Takarada; Mitsuaki Ono; Michinori Aoe; Wataru Kitamura; Masayuki Matsuda; Takashi Moriyama; Akifumi Matsumura; Yoshinobu Maeda
  • Journal Title: Cancer Science Volume: 115 Pages: 1317-1332
  • DOI: 10.1111/cas.16086
  • Peer Reviewed / Open Access
• [Journal Article] Exploring the Regulators of Keratinization: Role of BMP-2 in Oral Mucosa (2024)
  • Author(s): Xindi Mu; Mitsuaki Ono; Ha Thi Thu Nguyen; Ziyi Wang; Kun Zhao; Taishi Komori; Tomoko Yonezawa; Takuo Kuboki; Toshitaka Oohashi
  • Journal Title: Cells Volume: 13 Pages: 807
  • DOI: 10.3390/cells13100807
  • Peer Reviewed / Open Access
• [Journal Article] Lgr5-expressing secretory cells form a Wnt inhibitory niche in cartilage critical for chondrocyte identity (2023)
  • Author(s): Angela Ruscitto; Peng Chen; Ikue Tosa; Ziyi Wang; Gan Zhou; Ingrid Safina; Ran Wei; Mallory M. Morel; Alia Koch; Michael Forman; Gwendolyn Reeve; Michael K. Lecholop; Marshall Wilson; Daniel Bonthius; Mo Chen; Mitsuaki Ono; Timothy C. Wang;Hai Yao; Mildred Embree
  • Journal Title: Cell stem cell Volume: 30 Pages: 1179-1198
  • DOI: 10.1016/j.stem.2023.08.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ruxolitinib altered IFN-β induced necroptosis of human dental pulp stem cells during osteoblast differentiation (2023)
  • Author(s): Atsuko Tanaka; Satoru Hayano; Masayo Nagata; Takahiro Kosami; Ziyi Wang; Hiroshi Kamioka
  • Journal Title: Archives of Oral Biology Volume: 155 Pages: 10579
  • DOI: 10.1016/j.archoralbio.2023.105797
  • Peer Reviewed / Open Access
• [Presentation] Filamin A mediates embryonic palatal fusion by linking mechanotransduction with β-Catenin/Smad2. (2024)
  • Author(s): Ziyi Wang
  • Organizer: The 7th Research Meeting for International Students in Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University.
• [Presentation] A mechanosensor protein, Filamin A, mediated embryonic palate fusion through β-catenin/Smad2: A bioinformatics-oriented study. (2023)
  • Author(s): Ziyi Wang, Satoru Hayano, Yao Weng, Xindi Mu, Mitsuaki Ono, and Toshitaka Oohashi.
  • Organizer: 2023 ASBMR Annual Meeting
  • Int'l Joint Research