• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2022 Fiscal Year Annual Research Report

RNAノックダウンによるカイコ疾患モデルの開発と自然免疫機能の解明

Research Project

Project/Area Number 22F22741
Allocation TypeSingle-year Grants
Research InstitutionTeikyo University

Principal Investigator

関水 和久  帝京大学, 薬学部, 教授 (90126095)

Co-Investigator(Kenkyū-buntansha) YU YIDONG  帝京大学, 薬学部, 外国人特別研究員
Project Period (FY) 2022-09-28 – 2025-03-31
Keywordsbiodistribution of NPs / FANA ASOs in silkworms
Outline of Annual Research Achievements

Establishment of a fluorescent-signal based assay for quantifying biodistribution of nanoparticles (anionic liposomes labelled with rhodamine B) in different silkworm tissues following systemic administration.
Synthesis and characterization of anionic liposomes loaded with fluorescently labelled siRNA in Tokyo, following protocol provided by collaborator in Germany (University of Wuerzburg).
Quantification of RNA knockdown effects by FANA ASOs targeting selected genes (antimicrobial peptide-encoding genes) in primary silkworm cells (hemocytes) in vitro, followed by further evaluation in vivo (silkworms).

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

During the synthesis and characterization of anionic liposomes loaded with fluorescently labelled siRNA, it turned out that the encapsulation efficiency of siRNA in liposomes varied strongly among different batches, indicating that the protocol for synthesis should be further optimized to reduce batch variations (optimization by collaborator in Germany).

Strategy for Future Research Activity

Visualization of interaction between fluorescently labelled nanoparticles (with or without siRNA) and fungal pathogen in the silkworm.

Validating RNA knockdown effects of FANA ASOs on other selected target genes.

Visualization of fluorescently labelled FANA ASOs in silkworm tissues following different administration routes, for example, systemic vs. oral administration, to see how stable FANA ASOs are in silkworms, especially in the latter case.

  • Research Products

    (1 results)

All Other

All Int'l Joint Research (1 results)

  • [Int'l Joint Research] University of Wuerzburg(ドイツ)

    • Country Name
      GERMANY
    • Counterpart Institution
      University of Wuerzburg

URL: 

Published: 2023-12-25  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi