2022 Fiscal Year Annual Research Report
RNAノックダウンによるカイコ疾患モデルの開発と自然免疫機能の解明
| Project/Area Number |
22F22741
|
|---|
| Allocation Type | Single-year Grants |
|---|
| Research Institution | Teikyo University |
|---|
Principal Investigator |
関水 和久 帝京大学, 薬学部, 教授 (90126095)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YU YIDONG 帝京大学, 薬学部, 外国人特別研究員
|
|---|
| Project Period (FY) |
2022-09-28 – 2025-03-31
|
| Keywords | biodistribution of NPs / FANA ASOs in silkworms |
|---|
| Outline of Annual Research Achievements |
Establishment of a fluorescent-signal based assay for quantifying biodistribution of nanoparticles (anionic liposomes labelled with rhodamine B) in different silkworm tissues following systemic administration.
Synthesis and characterization of anionic liposomes loaded with fluorescently labelled siRNA in Tokyo, following protocol provided by collaborator in Germany (University of Wuerzburg).
Quantification of RNA knockdown effects by FANA ASOs targeting selected genes (antimicrobial peptide-encoding genes) in primary silkworm cells (hemocytes) in vitro, followed by further evaluation in vivo (silkworms).
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
During the synthesis and characterization of anionic liposomes loaded with fluorescently labelled siRNA, it turned out that the encapsulation efficiency of siRNA in liposomes varied strongly among different batches, indicating that the protocol for synthesis should be further optimized to reduce batch variations (optimization by collaborator in Germany).
|
| Strategy for Future Research Activity |
Visualization of interaction between fluorescently labelled nanoparticles (with or without siRNA) and fungal pathogen in the silkworm.
Validating RNA knockdown effects of FANA ASOs on other selected target genes.
Visualization of fluorescently labelled FANA ASOs in silkworm tissues following different administration routes, for example, systemic vs. oral administration, to see how stable FANA ASOs are in silkworms, especially in the latter case.
|
