2023 Fiscal Year Annual Research Report
| Project/Area Number |
22KJ0894
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| Allocation Type | Multi-year Fund |
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
韓 佩恂 国立研究開発法人理化学研究所, 開拓研究本部, 特別研究員(PD)
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Keywords | Ribosome |
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| Outline of Annual Research Achievements |
Translation, as a fundamental step in gene expression, requires extensive regulation. Recent studies have shown that the trigger of ribosome-associated quality control is the collision between two translating ribosomes. However, it is not clear whether endogenous mRNA sequences could also trigger quality control.
By deep-sequencing the ribosome footprints protected by collided ribosomes prior to rescue, we determined a specific feature in the mRNA sequence that contributed to its recognition by ribosome-associated quality control. Further analysis through reporter assay also validated our findings from the analysis.
These results combined provided crucial evidences regarding co-translational quality control pathways in higher eukaryotic cells.
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