2023 Fiscal Year Annual Research Report
Studies on CD4-2 positive cells a unique T cell subset of fish
| Project/Area Number |
22KJ1390
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| Allocation Type | Multi-year Fund |
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
Lau Lik Ming 東京海洋大学, 大学院海洋科学技術研究科, 特別研究員(DC1)
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Keywords | CD4-1 / CD4-2 / Ginbuna crucian carp / Cell-mediated immunity |
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| Outline of Annual Research Achievements |
In fiscal year (FY) 2023, in vivo transplantation of stimulated leukocytes from donor fish into recipient fish, and in vitro cytotoxicity assay of effector cells were studied using ginbuna as a model fish species. Through in vivo transplantation studies, I found that naive recipients who received stimulated leukocytes from peripheral blood and spleen of vaccinated donors tend to have a higher survival rate (90%) than those who received stimulated leukocytes from trunk-kidney and head-kidney (40%). These findings indicated that the majority of memory cells moved to a major secondary lymphoid organ such as the spleen and circulated in the systemic blood of ginbuna. Through in vitro cytotoxicity studies, regardless of stimulated or non-stimulated leukocytes, I realized that the cytotoxic activities of naive recipients who received donor cells with CD4-1 positive cells were higher than those of fish who received donor cells with CD4-2 positive cells. These results suggested that the adoptive transfer of stimulated leukocytes with CD4-1 positive cells rather than CD4-2 positive cells provided naive recipients efficient cytotoxic activity against virus-infected cells. However, naive recipients that received unsorted leukocytes from vaccinated donors following a secondary virus infection showed higher cytotoxic activities than those aforementioned treatment groups. These results suggested that secondary cell-mediated immunity is probably enhanced by CD4-1 and CD4-2 positive cells in ginbuna against a virus infection.
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