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2022 Fiscal Year Annual Research Report

Exploring natural analogues of future oceans to determine the adaptive potential of marine fishes to climate change

Research Project

Project/Area Number 22J20073
Allocation TypeSingle-year Grants
Research InstitutionOkinawa Institute of Science and Technology Graduate University

Principal Investigator

Izumiyama Michael  沖縄科学技術大学院大学, 科学技術研究科, 特別研究員(DC1)

Project Period (FY) 2022-04-22 – 2025-03-31
Keywordsenvironmental DNA / CO2 seep / climate change
Outline of Annual Research Achievements

During this fiscal year we conducted a sampling trip in collaboration with the researchers from the IRD (Research Institute for Development, France) collected fishes from mangrove lagoon in Borake New Caledonoia. This site is subjected to low pH during the outgoing tide which simulate predicted conditions of OA by the end of the century. We collected five species for a total of 149 fishes. The species collected include three species of damselfish Dascyllus aruanus, Chromis viridis, Pomacentrus moluccensis, and two species of cardinal fish Zoramia leptacantha, and Zoramia viridiventer. RNA was extracted from the brain and gills of the samples and sequenced and we are currently working on the data analysis. We also extracted DNA from 136 fin clips of Pomacentrus coelestis collected in and around a CO2 seep in Shikine, Japan to examine the population structure of fishes found at the seep. Results from environmental DNA (eDNA) collected at the Shikine CO2 seep was presented at the Australia & New Zealand Environmental DNA Conference. Hobart, Tasmania February 2023.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

The research progress has been proceeding as planned. We had to adjust our sampling scheme due to the pandemic but all sampling for this project has completed. We have sequenced all samples and we are working on analyzing the data for publication.

Strategy for Future Research Activity

Our plan is to present our results at two international conferences. We also plan on publishing our results this year.

  • Research Products

    (1 results)

All 2023

All Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] Community shifts at volcanic CO2 seeps, an eDNA prospective2023

    • Author(s)
      Michael Izumiyama
    • Organizer
      Australia & New Zealand Environmental DNA Conference
    • Int'l Joint Research

URL: 

Published: 2023-12-25  

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