Design and construction of new systems for regulating human cell fate

2014 Fiscal Year Final Research Report

• Project/Area Number: 23221011
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Chemical biology
• Research Institution: Kyoto University
• Principal Investigator: INOUE Tan 京都大学, 生命科学研究科, 教授 (40114855)
• Co-Investigator(Kenkyū-buntansha): SAITO Hirohide 京都大学, iPS細胞研究所, 教授 (20423014)
• Co-Investigator(Renkei-kenkyūsha): 樫田 俊一 京都大学, 大学院生命科学研究科 ; 遠藤 慧 京都大学, iPS細胞研究所, 博士研究員 (40626074) ; 藤田 祥彦 京都大学, 大学院生命科学研究科, 助教 (80192730) ; 原 知明 京都大学, 大学院生命科学研究科 ; 大野 博久 京都大学, 大学院生命科学研究科, 博士研究員 ; 佐川 文彦 京都大学, 大学院生命科学研究科, 技術員 ; 大内 将司 京都大学, 大学院生命科学研究科, 博士研究員 (20422412)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: RNP

Outline of Final Research Achievements

We developed new molecular switches for synthetic biology and bio-nanotechnology that respond to a specific protein in human cell. We also successfully incorporated feedback control of the switches and related techniques for the switches. A new general method for converting existing OFF molecular switches to the corresponding ON switches was developed.We designed and constructed a triangular shaped RNP with cell-surface receptor binding protein to control the signal transduction from the receptor. The RNP device is capable of regulating the signal transduction in an OFF and ON manner in human cell that regulate the apoptotic cell death. In addition, we also developed new methods for further advance our RNP technology.

Free Research Field

synthetic biology