2014 Fiscal Year Final Research Report
Development of novel biomarkers on exercise, stress and aging
| Project/Area Number |
23240097
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Waseda University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AKIMOTO Takayuki 東京大学, 大学院医学系研究科, 講師 (00323460)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Keywords | バイオマーカー / プロテオミクス / マイクロアレイ / リボソームプロファイリング |
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| Outline of Final Research Achievements |
In order to explore new biomarkers which respond to exercise and stress, we performed proteomics using blood and urine samples before and after exhaustive exercise and identified the molecules using mass spectrometric analyses. Among the molecules which exhibited remarkable increases, we developed measurement system using ELISA. Also, we identified several microRNAs in the blood circulation in response to acute resistant exercise using microarray, and quantified expression levels using real-time PCR. Furthermore, we set up global analyses of translation dynamics using ribosome profiling for biomarker discovery.
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| Free Research Field |
予防医学
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