Development of monitoring and remediation of persistent organic pollutants using constituents of xylem sap and root exudates from Cucurbitaceae family

2013 Fiscal Year Final Research Report

• Project/Area Number: 23241028
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Kobe University
• Principal Investigator: INUI Hideyuki 神戸大学, 遺伝子実験センター, 准教授 (90314509)
• Co-Investigator(Kenkyū-buntansha): EUN Heesoo (独)農業環境技術研究所, 有機化学物質研究領域, 主任研究員 (60343828) ; TSURUTA Hiroki 神戸大学, 連携創造本部, 准教授 (20346282)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 残留性有機汚染物質 / 植物 / 環境浄化 / 環境モニタリング / ダイオキシン / PCB / 導管液 / 根滲出物

Research Abstract

We tried to clarify uptake mechanisms of persistent organic pollutants, such as dioxins, by Cucurbitaceae family. Transporting proteins in xylem sap and organic acids in root exudates were responsible for its uptake to plants. Expression of genes related to uptake in plants led to dioxin accumulation in the aerial parts of plants. These results showed that phytoremediation systems for persistent pollutants are developed by the use of functions derived from Cucurbitaceae family.

Research Products

• [Journal Article] Molecular mechanisms of herbicide-inducible gene expression of tobacco CYP71AH11 metabolizing the herbicide chlorotoluron (2014)
  • Author(s): Gion, K., Inui, H., Takakuma, K., Yamada, T., Kambara, Y., Nakai, S., Fujiwara, H., Miyamura, T., Imaishi, H. and Ohkawa, H
  • Journal Title: Pesticide Biochemistry and Physiology Volume: 108 Pages: 49-57
  • DOI: 10.1016/j.pestbp.2013.12.003
  • Peer Reviewed
• [Journal Article] A major latex-like protein is a key factor in crop contamination by persistent organic pollutants (2013)
  • Author(s): Inui, H., Sawada, M., Goto, J., Yamazaki, K., Kodama, N., Tsuruta, H., Eun, H
  • Journal Title: Plant Physiology Volume: 161(4) Pages: 2128-2135
  • DOI: 10.1104/pp.112.213645
  • Peer Reviewed
• [Journal Article] Selective uptake of polychlorinated biphenyls by Cucurbita pepo (2012)
  • Author(s): Inui, H., Yamazaki, K., Matsuo, S., Yoshihara, R. and Eun, H
  • Journal Title: Organohalogen Compounds Volume: 74 Pages: 1213-1216
  • URL: http://www.dioxin20xx.org/pdfs/2012/1311.pdf
• [Journal Article] Assays of PCB congeners and organochlorine insecticides with the transgenic Arabidopsis and tobacco plants carrying recombinant guinea pig AhR and GUS reporter genes (2012)
  • Author(s): Gion, K., Inui, H., Sasaki, H., Utani, Y., Kodama, S. and Ohkawa, H
  • Journal Title: Journal of Environmental Science and Health, Part B : Pesticides, Food Contaminants, and Agricultural Wastes Volume: 47(7) Pages: 599-607
  • DOI: 10.1080/03601234.2012.668453
  • Peer Reviewed
• [Journal Article] Assays of dioxins and dioxin-like compounds in actually contaminated soils using transgenic tobacco plants carrying a recombinant mouse aryl hydrocarbon receptor-mediated ?-glucuronidase reporter gene expression system (2012)
  • Author(s): Inui, H., Gion, K., Utani, Y., Wakai, T., Kodama, S., Eun, H., Kim, Y.-S. and Ohkawa, H
  • Journal Title: Journal of Environmental Science and Health, Part B : Pesticides, Food Contaminants, and Agricultural Wastes Volume: 47(1) Pages: 59-65
  • DOI: 10.1080/03601234.2012.611018
  • Peer Reviewed
• [Journal Article] Structure-selective accumulation of polychlorinated biphenyls in Cucurbita pepo (2011)
  • Author(s): Matsuo, S., Yamazaki, K., Gion, K., Eun, H. and Inui, H
  • Journal Title: Journal of Pesticide Science Volume: 36(3) Pages: 363-369
  • DOI: 10.1584/jpestics.G11-03
  • Peer Reviewed
• [Journal Article] Congener specificity in the accumulation of dioxins and dioxin-like compounds in zucchini plants grown hydroponically (2011)
  • Author(s): Inui, H., Wakai, T., Gion, K., Yamazaki, K., Kim, Y.-S. and Eun, H
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75(4) Pages: 705-710
  • DOI: 10.1271/bbb.100833
  • Peer Reviewed
• [Journal Article] Structural basis of species differences between human and experimental animal CYP1A1s in metabolism of 3,3',4,4',5-pentachlorobiphenyl (2011)
  • Author(s): Yamazaki, K., Suzuki, M., Itoh, T., Yamamoto, K., Kanemitsu, M., Matsumura, C., Nakano, T., Sakaki, T., Fukami, Y., Imaishi, H. and Inui, H
  • Journal Title: Journal of Biochemistry Volume: 149(4) Pages: 487-494
  • DOI: 10.1093/jb/mvr009
  • Peer Reviewed
• [Journal Article] Phytomonitoring and phytoremediation of agrochemicals and related compounds based on recombinant cytochrome P450s and aryl hydrocarbon receptors (AhRs) (2011)
  • Author(s): Shimazu, S., Inui, H. and Ohkawa, H
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 59 Pages: 2870-2875
  • DOI: 10.1021/jf102561d
  • Peer Reviewed
• [Presentation] 各種ウリ科植物が持つ残留性有機汚染物質の体内輸送因子の比較 (2014)
  • Author(s): 岩渕彩、後藤純弥、乾秀之
  • Organizer: 第23回環境化学討論会
  • Place of Presentation: 京都
  • Year and Date: 2014-05-16
• [Presentation] ウリ科植物の根における脂溶性物質結合タンパク質の局在性 (2014)
  • Author(s): 吉原亮平、後藤純弥、岩渕彩、池田健一、井上加奈子、乾秀之
  • Organizer: 2014年度日本農芸化学会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-29
• [Presentation] ウリ科植物における残留性有機汚染物質の体内輸送因子の機能・動態 (2014)
  • Author(s): 後藤純弥、岩渕彩、乾秀之
  • Organizer: 2014年度日本農芸化学会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-28
• [Presentation] ズッキーニにおけるダイオキシン類の取り込みに係わる遺伝子の機能解析 (2014)
  • Author(s): 乾秀之、廣田又士、後藤純弥、吉原亮平、兒玉典子、松井智美、山﨑清志、殷煕洙
  • Organizer: 2014年度日本農芸化学会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-28
• [Presentation] ウリ科植物における残留性有機汚染物質の取り込みメカニズム (2014)
  • Author(s): 乾秀之、沢田真美、後藤純弥、山﨑清志、兒玉典子、鶴田宏樹、殷熙洙
  • Organizer: 日本農薬学会第39回大会
  • Place of Presentation: 京都
  • Year and Date: 2014-03-15
• [Presentation] ウリ科植物が持つダイオキシン類蓄積性能に係わる因子の同定 (2014)
  • Author(s): 廣田又士、後藤純弥、吉原亮平、殷熙洙、乾秀之
  • Organizer: 日本農芸化学会関西支部第483回講演会
  • Place of Presentation: 京都
  • Year and Date: 2014-02-01
• [Presentation] Amino acid variations in mammalian cytochrome P450 monooxygenases responsible for decrease of dioxin-like toxicity of polychlorinated biphenyls (2013)
  • Author(s): Hideyuki Inui, Kiyoshi Yamazaki, Miku Yabu, Toshimasa Itoh, Keiko Yamamoto, Motoharu Suzuki, Yuuki Haga, Shintarou Mise, Masahiro Tsurukawa, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki
  • Organizer: 33nd International Symposium on Halogenated Persistent Organic Pollutants
  • Place of Presentation: Daegu, Korea
  • Year and Date: 20130828-29
• [Presentation] Structural Basis of Species Differences between Rat and Human CYP1A1s in Metabolism of Polychlorinated Biphenyls (2013)
  • Author(s): Hideyuki Inui
  • Organizer: 22nd Symposium on Environmental Chemistry
  • Place of Presentation: Mini-symposium, Tokyo
  • Year and Date: 2013-08-02
• [Presentation] ラットおよびヒト由来シトクロムP450 モノオキシゲナーゼによるCB118の代謝と3次元構造の比較 (2013)
  • Author(s): 見世慎太朗、羽賀雄紀、薮未来、伊藤俊将、山本恵子、鶴川正寛、松村千里、中野武、榊利之、乾秀之
  • Organizer: 第22回環境化学討論会
  • Place of Presentation: 東京
  • Year and Date: 2013-08-02
• [Presentation] Hydrophilic and hydrophobic pathways for the uptake of compounds in lateral roots based on in planta observation (2013)
  • Author(s): Kiyoshi Yamazaki, Hiroki Tsuruta, Hideyuki Inui
  • Organizer: International Workshop on Plant Membrane Biology XVI
  • Place of Presentation: Kurashiki
  • Year and Date: 2013-03-28
• [Presentation] In planta 観察法を用いた根における親水性及び疎水性化合物の取り込み経路の解明 (2013)
  • Author(s): 山﨑清志、鶴田宏樹、乾秀之
  • Organizer: 日本農薬学会第38回大会
  • Place of Presentation: つくば
  • Year and Date: 2013-03-16
• [Presentation] ポリ塩化ビフェニル代謝能に影響を及ぼすラットおよびヒトCYP1A1 の基質結合部位を構成するアミノ酸の同定 (2012)
  • Author(s): 薮未来、羽賀雄紀、山﨑清志、鈴木元治、伊藤俊将、山本恵子、鶴川正寛、松村千里、中野武、榊利之、乾秀之
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-15
• [Presentation] Structural basis of species differences between rat and human CYP1A1s in metabolism of polychlorinated biphenyls (2012)
  • Author(s): Miku Yabu, Kiyoshi Yamazaki, Motoharu Suzuki, Yuki Haga, Toshimasa Itoh, Keiko Yamamoto, Masahiro Tsurukawa, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, and ○Hideyuki Inui
  • Organizer: チトクロムP450 発見50周年記念シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-03
• [Presentation] ウリ科植物に学ぶ脂溶性汚染物質の環境浄化メカニズム (2012)
  • Author(s): 乾秀之
  • Organizer: 創造例会2012、はりま産学交流会
  • Place of Presentation: 姫路
  • Year and Date: 2012-11-15
• [Presentation] ウリ科植物に学ぶ脂溶性汚染物質の環境浄化メカニズム (2012)
  • Author(s): 乾秀之
  • Organizer: 第10回積水化学自然に学ぶものづくりフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2012-10-15
• [Presentation] Crucial factors in species differences on metabolism of polychlorinated biphenyls by rat and human CYP1A1s (2012)
  • Author(s): Miku Yabu, Kiyoshi Yamazaki, Motoharu Suzuki, Yuki Haga, Toshimasa Ito, Keiko Yamamoto, Masahiro Tsurukawa, Chisato Matsumura, Takeshi Nakano, Toshiyuki Sakaki, and Hideyuki Inui
  • Organizer: SETAC Asia/Pacific 2012
  • Place of Presentation: Kumamoto
  • Year and Date: 2012-09-26
• [Presentation] High accumulation and congener specific uptake of dioxins and dioxin-like compounds in Cucurbita pepo (2012)
  • Author(s): Ryouhei Yoshihara, Kiyoshi Yamazaki, Heesoo Eun, Hideyuki Inui
  • Organizer: SETAC Asia/Pacific 2012
  • Place of Presentation: Kumamoto
  • Year and Date: 2012-09-24
• [Presentation] Selective uptake of polychlorinated biphenyls by Cucurbita pepo (2012)
  • Author(s): Hideyuki Inui, Kiyoshi Yamazaki, Shinryo, Matsuo, Ryouhei Yoshihara, Heesoo Eun
  • Organizer: 32nd International Symposium on Halogenated Persistent Organic Pollutants
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2012-08-30
• [Presentation] 残留性有機汚染物質によるウリ科植物の汚染を引き起こす遺伝子の同定 (2012)
  • Author(s): 廣田又士、吉原亮平、兒玉典子、殷熙洙、山﨑清志、乾秀之
  • Organizer: 第21回環境化学討論会
  • Place of Presentation: 松山
  • Year and Date: 2012-07-13
• [Presentation] ズッキーニによるダイオキシン類の取り込みに係わる遺伝子の同定 (2012)
  • Author(s): 乾秀之、廣田又士、山﨑清志
  • Organizer: 2012年度日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-25
• [Presentation] 植物の根による脂溶性化合物の吸収経路 (2012)
  • Author(s): 山﨑清志、鶴田宏樹、乾秀之
  • Organizer: 日本農薬学会第37回大会
  • Place of Presentation: 岡山
  • Year and Date: 2012-03-16
• [Presentation] Congener specificity in the accumulation of dioxins and dioxin-like compounds in Cucurbita pepo grown hydroponically (2011)
  • Author(s): Hideyuki Inui, Taketo Wakai, Keiko Gion, Kiyoshi Yamazaki, Yun-Seok Kim, and Heesoo Eun
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference
  • Place of Presentation: Kobe
  • Year and Date: 2011-11-30
• [Presentation] Accumulation mechanism of dioxins and dioxin-like compounds in Cucurbita pepo (2011)
  • Author(s): Kiyoshi Yamazaki, Shinryo Matsuo, Heesoo Eun, Hiroki Tsuruta, Hideyuki Inui
  • Organizer: 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference
  • Place of Presentation: Kobe
  • Year and Date: 2011-11-29
• [Presentation] ラットおよびヒトCYP1A1 によるポリ塩化ビフェニルの代謝と3次元構造の比較 (2011)
  • Author(s): 薮未来、山﨑清志、鈴木元治、羽賀雄紀、伊藤俊将、山本恵子、鶴川正寛、松村千里、中野武、榊利之、乾秀之
  • Organizer: 第20回環境化学討論会
  • Place of Presentation: 熊本
  • Year and Date: 2011-07-16
• [Presentation] Cucurbita pepo におけるダイオキシン類の器官局在性 (2011)
  • Author(s): 松井智美、松尾申遼、殷熙洙、山﨑清志、乾秀之
  • Organizer: 第20回環境化学討論会
  • Place of Presentation: 熊本
  • Year and Date: 2011-07-16
• [Book] 1-7バレイショの形質転換プロトコール、形質転換プロトコール【植物編】、田部井豊編 (2012)
  • Author(s): 乾秀之
  • Total Pages: 64-70
  • Publisher: (株)化学同人
• [Book] 植物機能のポテンシャルを活かした環境保全・浄化技術ー地球を救う超環境適合・自然調和型システムー、第2章ファイトレメディエーション:植物による土壌浄化技術、6 残留農薬のファイトレメディエーションとポリ塩化ビフェニル(PCB)などのファイトモニタリング、池道彦、平田收正編 (2011)
  • Author(s): 大川秀郎、乾秀之、嶋津小百合
  • Total Pages: 92-99
  • Publisher: (株)シーエムシー出版
• [Remarks] 神戸大学 遺伝子実験センター 環境遺伝子機能解析研究分野 乾グループ
  • URL: http://www.research.kobe-u.ac.jp/rceg/doc./inui/index.html