Modification of cellular function based on cell fusion in microdevices

2014 Fiscal Year Final Research Report

• Project/Area Number: 23241041
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Microdevices/Nanodevices
• Research Institution: The University of Tokyo
• Principal Investigator: WASHIZU MASAO 東京大学, 工学(系)研究科(研究院), 教授 (10201162)
• Co-Investigator(Kenkyū-buntansha): KOTERA Hidetoshi 京都大学, 大学院工学研究科, 教授 (20252471)
• Co-Investigator(Renkei-kenkyūsha): OANA Hidehiro 東京大学, 大学院工学系研究科, 准教授 (20314172) ; GEL Murat 東京大学, 大学院工学系研究科, 助教 (90456153)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: ナノ・マイクロ科学 / 細胞融合 / 初期化 / 再生医療 / 静電気 / ES細胞 / 微細加工 / マイクロ・ナノデバイス

Outline of Final Research Achievements

We have successfully established a novel cell fusion method based on field constriction created by microorifices. Using the method, fusion among ES-cells and tissue cells are performed, which resulted in bistable flat- and domelike- colony, which in 7 days showed typical colony shape of initialized cells. Another sample shown the initialization reporter from the colony. We have also shown the fusion of B cells and myeloma cell, which resulted in the hybridoma colony with the very high yield of 5%. These results show that our high-yield fusion method opens a way for cell initialization, as well as high yield production of mono-clonal antibodies.

Free Research Field

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