2013 Fiscal Year Final Research Report
Genome evolution induced by chromatin structure and DNA methylation
Project/Area Number |
23241058
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Genome biology
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Research Institution | The University of Tokyo |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
TAKEDA Hiroyuki 東京大学, 大学院理学系研究科, 教授 (80179647)
HASHIMOTO Shinichi 金沢大学, 医学系, 特任教授 (00313099)
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Project Period (FY) |
2011-05-31 – 2014-03-31
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Keywords | DNAメチル化 / 塩基置換 / 進化 / 近交系 / ヒトゲノム / メダカゲノム |
Research Abstract |
Natural selection and neutral theory of evolution have been crucial to understand genetic variation and evolution. In this study, we have examined whether chromatin structure and DNA methylation might affect genetic variation and evolution. Indeed, we identified an unexpected genome-wide role of the CpG methylation state as a major determinant of proximal natural genetic variation. ~18% of the human genome was within 10 bp of a CpG site; in these regions, the single nucleotide polymorphism rate significantly increased by ~50% (P<E-566) if the neighboring CpG sites are methylated. In two medaka inbred strains, we observed a distinctive ~200-base pair periodic pattern of genetic variation downstream of transcription start sites and its correlation with the chromatin structure. These data exemplify the potential for genetic activity (transcription), DNA methylation, and chromatin structure to contribute to molding the DNA sequence on an evolutionary time scale.
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[Journal Article] Coordinated changes in DNA methylation in antigen-specific memory CD4 T cells2013
Author(s)
Hashimoto S, Ogoshi K, Sasaki A, Abe J, Qu W, Nakatani Y, Ahsan B, Oshima K, Shand FH, Ametani A, Suzuki Y, Kaneko S, Wada T, Hattori M, Sugano S, Morishita S, Matsushima K
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Journal Title
J Immunol
Volume: 190(8)
Pages: 4076-91
DOI
Peer Reviewed
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[Journal Article] Genome-wide genetic variations are highly correlated with proximal DNA methylation patterns2012
Author(s)
Qu W, Hashimoto S, Shimada A, Nakatani Y, Ichikawa K, Saito TL, Ogoshi K, Matsushima K, Suzuki Y, Sugano S, Takeda H, Morishita S
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Journal Title
Genome Research
Volume: 22(8)
Pages: 1419-25
DOI
Peer Reviewed
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