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2014 Fiscal Year Final Research Report

Synthesis of 8-substituted purine chemical library and riboswitch-reengineering

Research Project

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Project/Area Number 23241073
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemical biology
Research InstitutionOsaka University

Principal Investigator

NAKATANI Kazuhiko  大阪大学, 産業科学研究所, 教授 (70237303)

Co-Investigator(Kenkyū-buntansha) TAKEI Fumie  大阪大学, 産業科学研究所, 助教 (30252711)
HAGIHARA Masaki  弘前大学, 理工学研究科, 准教授 (40403000)
Research Collaborator DOHNO Chikara  大阪大学, 産業科学研究所, 准教授 (60420395)
MURATA Asako  大阪大学, 産業科学研究所, 助教 (50557121)
FUKUZUMI Takeo  大阪大学, 産業科学研究所, 特任助教 (00592768)
AIKAWA Haruo  大阪大学, 産業科学研究所, 特任助教 (70547322)
Project Period (FY) 2011-04-01 – 2015-03-31
Keywordssynthesis / 8-substituted purine / chemical library / riboswitch / reengineering
Outline of Final Research Achievements

In the projet "Synthesis of 8-substituted purine chemical library and ribolswitch-reengineering", we have focused our attention on the synthesis of compound library of 8-substituted purim derivatives, and the application of compounds for the regulation of artificial riboswitches. The synthesis of 8-substituted purine derivatives were successfully done by a coupling of 8-bromo-adenine and 8-bromo-adenosine with the commercially available borate esters under Suzuki-Miyaura coupling conditions, providing us a chemical library of 8-substituted purines. With these compounds in hand, we looked for the RNA functioning as riboswiches by in vitro selection using doped library of adenine riboswitch. We have changed the selection conditions, the desired RNA functioning as riboswitch could not be selected. The lack of diversity of RNA sequence was one of reason for these results.

Free Research Field

ケミカルバイオロジー

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Published: 2016-06-03  

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