Synthesis of 8-substituted purine chemical library and riboswitch-reengineering

2014 Fiscal Year Final Research Report

• Project/Area Number: 23241073
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Osaka University
• Principal Investigator: NAKATANI Kazuhiko 大阪大学, 産業科学研究所, 教授 (70237303)
• Co-Investigator(Kenkyū-buntansha): TAKEI Fumie 大阪大学, 産業科学研究所, 助教 (30252711) ; HAGIHARA Masaki 弘前大学, 理工学研究科, 准教授 (40403000)
• Research Collaborator: DOHNO Chikara 大阪大学, 産業科学研究所, 准教授 (60420395) ; MURATA Asako 大阪大学, 産業科学研究所, 助教 (50557121) ; FUKUZUMI Takeo 大阪大学, 産業科学研究所, 特任助教 (00592768) ; AIKAWA Haruo 大阪大学, 産業科学研究所, 特任助教 (70547322)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: synthesis / 8-substituted purine / chemical library / riboswitch / reengineering

Outline of Final Research Achievements

In the projet "Synthesis of 8-substituted purine chemical library and ribolswitch-reengineering", we have focused our attention on the synthesis of compound library of 8-substituted purim derivatives, and the application of compounds for the regulation of artificial riboswitches. The synthesis of 8-substituted purine derivatives were successfully done by a coupling of 8-bromo-adenine and 8-bromo-adenosine with the commercially available borate esters under Suzuki-Miyaura coupling conditions, providing us a chemical library of 8-substituted purines. With these compounds in hand, we looked for the RNA functioning as riboswiches by in vitro selection using doped library of adenine riboswitch. We have changed the selection conditions, the desired RNA functioning as riboswitch could not be selected. The lack of diversity of RNA sequence was one of reason for these results.

Free Research Field

ケミカルバイオロジー