2013 Fiscal Year Final Research Report
Methanogenesis under high pressure conditions mimicking subsurface environments
| Project/Area Number |
23244109
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Geochemistry/Astrochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KAMAGATA YOICHI 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究部門長 (70356814)
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| Co-Investigator(Kenkyū-buntansha) |
SAKATA Susumu 産業技術総合研究所, 地圏資源環境部門研究, グループ長 (70357101)
吉岡 秀佳 産業技術総合研究所, 地圏資源環境部門, 主任研究員 (30415765)
皆川 秀紀 産業技術総合研究所, メタンハイドレート研究センター, 研究チーム長 (70202348)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | メタン生成菌 / メタンハイドレート / 天然ガス / 高圧培養 / 微生物間共生 / 地下圏微生物 / 安定同位体比 / 水素 |
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| Research Abstract |
We investigated whether biological methanogensis occurs under high pressure conditions (mimicking subsurface environments). We used pure cultures of hydrogenotrophic and aceticlastic methanogens, and an acetate oxidizing hydrogen producing syntroph as well as environmental subsurface samples taken from an oil field. We obtained the following results: 1) pure cultures of hydrogenotrophic and aceticlastic methanogens, and syntrophic coculture were successfully cultivated under high pressure conditions (up to 15MPa). Note that the syntrophic coculture grew and produced methane under such high pressure conditions. 2) Methoxylated benzoates were used as model kerogen derivatives to enrich communities that were capable of degrading them to produce methane. 3) In the acetate-utilizing methane-producing community, CO2 concentration is the crucial factor driving either aceticlastic methanogenesis or syntrophic acetate oxidation and subsequent methanogenesis from H2 and CO2.
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