2013 Fiscal Year Final Research Report
Chemical Synthesis of glycoproteins having complex type oligosaccharides and elucidation of oligosaccharide functions
| Project/Area Number |
23245037
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
KAJIHARA Yasuhiro 大阪大学, 理学(系)研究科(研究院), 教授 (50275020)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 糖鎖 / 糖タンパク質 |
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| Research Abstract |
In order to elucidate oligosaccharide functions, chemical synthesis of homogeneous glycoprotein was examined. For the synthesis of target glycoproteins, full-length glycosylpolypeptide chains was designed to divide into several segments and these were prepared by our improved and safe tert-Boc-SPPS. Glycopeptide-alpha-thioester and peptide-alpha-thioesters thus obtained were successfully coupled by repetitive native chemical ligation. Subsequent folding experiments of chemically synthesized homogeneous glycopeptide yielded correctly folded glycoproteins with native disulfide bond patterns. Using this method, erythropoietin, chemokine and lectin were successfully synthesized.
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