Laser sintering of tissue engineering scaffold for reconstruction of three dimensional, large scale and high metabolic rate organs

2014 Fiscal Year Final Research Report

• Project/Area Number: 23246027
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Production engineering/Processing studies
• Research Institution: The University of Tokyo
• Principal Investigator: NIINO TOSHIKI 東京大学, 生産技術研究所, 教授 (70291929)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: additive manufacturing / tissue engineering / laser sintering / 3D printing

Outline of Final Research Achievements

Tissue engineering is basically composed of the three technologies which are concerning cells, their culture and scaffold. This research is concerning the fabrication of tissue engineering scaffold with laser sintering, one of so-called 3D printer technology. In this research, short wave length of 1.1 microns was selected to reduce the laser spot diameter, while typical commerciall systems employ 11microns, and a prototype scaffold was successfully developed out of typical scaffold material of Polycaprolactone. Fabrication technique that reduces powder bed temperature was developed. Material of porogen powder was switched from sodium chloride to less heat conductive and alkaline soluble plastic. Owing two these developments, precision of scaffold was roughly doubled. Hydrophilicity treatment of PCL scaffold with alkaline solution was established to improve cell adhesion. Resultantly, stability of cell culture of HepG2 cells was improved.

Free Research Field

生産加工