The morphogenesis and functional differentiation of endoplasmic reticulum in plant cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 23247009
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: University of Hyogo
• Principal Investigator: SHIMMEN Teruo 兵庫県立大学, 生命理学研究科, 教授 (80114510)
• Co-Investigator(Kenkyū-buntansha): 横田 悦雄 兵庫県立大学, 生命理学研究科, 助教 (80212299)
• Co-Investigator(Renkei-kenkyūsha): 西村 いくこ 京都大学, 理学研究科, 教授 (00241232) ; 園部 誠司 兵庫県立大学, 生命理学研究科, 准教授 (30197024)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 小胞体 / ＲＨＤ３ / ＧＴＰ / シロイヌナズナ / ミオシン

Outline of Final Research Achievements

We investigated the role of RHD3, a plant homologue of atlastin GTPase, on the membrane fusion of endoplasmic reticulum. Based on analyses of the membrane fusion and the tubular formation in vitro of endoplasmic reticulum isolated from Arabidopsis seedling or cultured cell, RHD3 was revealed to be involved in GTP-dependent membrane fusion of endoplasmic reticulum. Furthermore, the biochemical approach with the chemical cross-linkers showed that the membrane fusion of endoplasmic reticulum was progressed through the formation of RHD3 oligomer by the association with GTP.

Free Research Field

植物生理学