2013 Fiscal Year Final Research Report
Understanding of molecular mechanism of the assembly of two RecA homologs, Rad51 and Dmc1
| Project/Area Number |
23247029
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 組換え / 減数分裂 / ゲノム安定化 / Rad51 |
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| Outline of Final Research Achievements |
During recombination, homology search between two DNA molecules is a key reaction which is mediated by the RecA homolog Rad51 during mitosis. On the other hand, homology search in meiotic recombination is catalyzed by a collaborative action of two RecA homolog, Rad51 and Dmc1. Both Rad51 and Dmc1 form a filament on the single-stranded DNAs which is regulated in a positive and negative way. We identified a new protein complex containing Psy3, Csm2, Shu1 and Shu2 for Rad51 assembly. We determined a crystal structure of a core component of this complex, Psy3-Csm2. Surprisingly, Psy3-Csm2 shows a structural similarity to Rad51 dimer, although there is little sequence similarity between the proteins. Based on the structure, we propose how the Psy3-Csm2 promotes Rad51 filament on the DNA. This is very novel finding to understand molecular mechanism on how Rad51 mediators facilitate Rad51 filament formation.
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| Free Research Field |
基礎生物学
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