Molecular structures, functional coordination, and cell dynamics of vacuolar proton pump and metal transporters

2013 Fiscal Year Final Research Report

• Project/Area Number: 23248017
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: MAESHIMA Masayoshi 名古屋大学, 生命農学研究科, 教授 (80181577)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 金属イオン / プロトンポンプ / イオン輸送体 / 液胞 / 分子構造 / 機能調節

Research Abstract

We focussed our attention to the vacuolar proton H+-PPase and zinc transporter MTP1 and obtained the following information. (1) Individual biochemical role of 30 residues of MTP1 (FEBS J., 2012), (2) structural and ligand binding property of the His-rich loop of MTP1 (FEBS Open Bio, 2013), (3) biochemical dynamics of membrane transporters under Zn stress (Plant Physiol., 2011), functional role of H+-PPase for early development of seedlings (Plant Cell 2011, Plant Signal. Behav. 2012), and biochemical significance of interaction of H+-PPase and phosphatidylinositol phosphates (Plant Cell 2013).

Research Products

• [Journal Article] The ABCA9 transporter facilitates seeds storage lipid synthesis at the endoplasmic reticulum (2013)
  • Author(s): Kim, S., Yamaoka, Y., Ono, H., Kim, H., Shim, D., Maeshima, M., Martinoia, E., Cahoon, E.B., Nishida, I., and Lee, Y.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 110 Pages: 773-778
  • Peer Reviewed
• [Journal Article] Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane (2013)
  • Author(s): Wang, B., Bailly, A., Zwiewka, M., Henrichs, S., Azzarello, E., Mancuso, S., Maeshima, M., Friml, J., Schulz, A., and Geisler, M.
  • Journal Title: Plant Cell Volume: 25 Pages: 202-214
  • Peer Reviewed
• [Journal Article] A Ca2+-binding protein PCaP2 located on the plasma membrane is involves in root hair development as a possible signal transducer (2013)
  • Author(s): Kato, M., Aoyama, T., and Maeshima, M.
  • Journal Title: Plant J. Volume: 74 Pages: 690-700
  • Peer Reviewed
• [Journal Article] Zinc-binding and structural properties of the histidine-rich loop of Arabidopsis thaliana vacuolar membrane zinc transporter MTP1 (2013)
  • Author(s): Tanaka, N., Kawachi, M., Fujiwara, T., and Maeshima, M.
  • Journal Title: FEBS Open Bio Volume: 3 Pages: 218-224
  • Peer Reviewed
• [Journal Article] A role of phosphatidylinositol 3,5-bisphosphate in vacuolar structure change in guard cells of closing stomata (2013)
  • Author(s): Bak, G., Lee, E.J., Lee, Y., Kato, M., Segami, S., Heven, S., Maeshima, M., Hwang, J.U., and Lee, Y.
  • Journal Title: Plant Cell Volume: 25 Pages: 2202-2216
  • Peer Reviewed
• [Journal Article] Local distribution of membrane proteins on vacuoles isolated from Arabidopsis suspension-cultured cells (2013)
  • Author(s): Yoshida, K., Ohnishi, M., Fukao, Y., Okazaki, Y., Song, C., Hayashi, F., Fujiwara, M., Nakanishi, Y., Saito, K., Shimmen, T., Suzaki, T., Fukaki, H., Maeshima, M., and Mimura, T.
  • Journal Title: Plant Cell Physiol. Volume: 54 Pages: 1571-1584
  • Peer Reviewed
• [Journal Article] Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family (2013)
  • Author(s): Chen, Z., Fujii, Y., Yamaji, N., Masuda, S., Takemoto, Y., Kamiya, T., Yusuyin, Y., Iwasaki, K., Kato, S., Maeshima, M., Ma, J.F., and Ueno, D.
  • Journal Title: J. Exp. Botany Volume: 64 Pages: 4375-4387
  • Peer Reviewed
• [Journal Article] Enhanced cell expansion in KRP2 overexpressor is mediated through increased V-ATPase activity (2013)
  • Author(s): Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Cell Physiol. Volume: 54 Pages: 1989-1998
  • Peer Reviewed
• [Journal Article] Class III compensation, represented by KRP2 overexpression, depends on V-ATPase activity in proliferative cells (2013)
  • Author(s): Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Signaling and Behavior Volume: 8 Pages: e27204
  • Peer Reviewed
• [Journal Article] Regulation of pyrophosphate levels by the H+-PPase is central for proper resumption of early plant development (2012)
  • Author(s): Ferjani, A., Segami, S., Horiguchi, G., Sakata, A., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Signaling & Behavior Volume: 7 Pages: 38-42
  • Peer Reviewed
• [Journal Article] Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement (2012)
  • Author(s): Vijayapalani, P., Maeshima, M., Nagasaki-Takekuchi, N., and Miller, W.A.
  • Journal Title: PLoS Pathogens Volume: 8 Pages: e1002639
  • Peer Reviewed
• [Journal Article] Amino acid screening based on structural modeling identifies critical residues for the function, ion selectivity and structure of Arabidopsis MTP1 (2012)
  • Author(s): Kawachi, M., Kobae, Y., Kogawa, S., Mimura, T., Kramer, U., and Maeshima, M.
  • Journal Title: FEBS J. Volume: 279 Pages: 2339-2356
  • Peer Reviewed
• [Journal Article] Stimulation of root growth induced by aluminum in Quercus serrata Thunb. is related to activity of nitrate reductase and maintenance of IAA concentration in roots (2012)
  • Author(s): Tomioka, R., Takenaka, C., Maeshima, M., and Tezuka, T., Kojima, M., Sakakibara, H.
  • Journal Title: American J. Plant Sciences Volume: 3 Pages: 1619-1624
  • Peer Reviewed
• [Journal Article] Vacuolar proton pumps and aquaporins involved in rapid internode elongation of deepwater rice (2011)
  • Author(s): Muto, Y., Segami, S., Hayashi, H., Sakurai, J., Murai, M., Hattori, Y., Ashikari, M., and Maeshima, M.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 75 Pages: 114-122
  • Peer Reviewed
• [Journal Article] The iTRAQ analysis reveals mechanisms of growth defects due to excess zinc in Arabidopsis thaliana (2011)
  • Author(s): Fukao, Y., Ferjani, A., Tomioka, R., Nagasaki, N., Kurata, R., Nishimori, Y., Fujiwara, M., and Maeshima, M.
  • Journal Title: Plant Physiology Volume: 155 Pages: 1893-1907
  • Peer Reviewed
• [Journal Article] Chilling-induced changes of vacuolar proton pumps of hypocotyls from Vigna unguiculata (2011)
  • Author(s): Oliveira, L.M.N., Sobreira, A.C., Sobreira, A.C.M., Costa, J.H., Otoch, M.L.O., Maeshima, M., and Fernandes de Melo, D.
  • Journal Title: Plant Growth Regulation Volume: 64 Pages: 211-219
  • Peer Reviewed
• [Journal Article] Keep an eye on PPi : The vacuolar-type H+-pyrophosphatase regulates postgerminative development in Arabidopsis (2011)
  • Author(s): Ferjani, A., Segami, S., Horiguchi, G., Muto, Y., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Cell Volume: 23 Pages: 2895-2908
  • Peer Reviewed
• [Journal Article] The small GTPase Rab5a is essential for intracellular transport of proglutelin from Golgi apparatus to the protein storage vacuole and endosomal membrane organization in developing rice endosperm (2011)
  • Author(s): Fukuda, M., Satoh-Cruz, M., Wen, L., Crofts, A.J., Sugino, A., Washida, H., Okita, T.W., Ogawa, M., Y. Kawagoe, Y., Maeshima, M., and Kumamaru, T.
  • Journal Title: Plant Physiol. Volume: 157 Pages: 632-644
  • Peer Reviewed
• [Presentation] Dimerization of GFPs fused to H+-pyrophosphatase influences the morphology and dynamics of vacuoles (2013)
  • Author(s): Segami, S. et al.
  • Organizer: International Workshop on Plant Membrane Biology
  • Place of Presentation: Okayama
  • Year and Date: 20130326-31
• [Book] Regulation of PPi levels through vacuolar membrane H+-pyrophosphatase. In Progress in Botany, Vol.75, ed. Luttge, Beyschlag and Cushman (2013)
  • Author(s): Ferjani, A., Segami, S., Asaoka, M. and Maeshima, M.
  • Total Pages: 145-166
  • Publisher: Springer-Verlag, Heidelberg
• [Book] The role of membrane transport in the detoxification and accumulation of zinc in plants. In A Soil Biology Series: Detoxification of Heavy Metals (2011)
  • Author(s): Kawachi, M., Kobae, Y., Tomioka, R., and Maeshima, M.
  • Total Pages: 129-142
  • Publisher: Springer-Verlag
• [Book] Application of radioisotope in biochemical analysis: Metal binding proteins and metal transporters. In Radioisotopes - Applications in Bio-Medical Science, Ed by Singh, N. (2011)
  • Author(s): Kawachi, M., Nagasaki-Takeuchi, N., Kato, M., and Maeshima, M.
  • Total Pages: 115-126
  • Publisher: InTech
• [Remarks]
  • URL: http://celld.agr.nagoya-u.ac.jp/index.html