Mechanisms for phase shift of reactivated memory

2013 Fiscal Year Final Research Report

• Project/Area Number: 23300120
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: KIDA Satoshi 東京農業大学, 応用生物科学部, 教授 (80301547)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 神経科学 / 脳・神経 / 記憶再固定化 / 記憶消去 / 記憶想起 / 記憶固定化 / 海馬 / 扁桃体

Research Abstract

Memory retrieval initiates two opposite processes; memory reconsolidation and extinction. Memory reconsolidation maintains or enhances fear memory, whereas memory extinction weakens fear memory. In this study, we tried to understand mechanisms by which the fate of memory is determined after retrieval by analyzing activation of transcription factor CREB, ERK and proteasome-dependent protein degradation in the hippocampus, amygdala and mPFC. We identified reconsolidation neurons and extinction neurons that display distinct biochemical features in reconsolidation and extinction phases, respectively. More importantly, in the extinction phase, reconsolidation neurons were inactivated while extinction neurons activated ERK signaling pathway in the hippocampus. From these observations, we concluded that hippocampus plays key roles in the phase shift from reconsolidation to extinction following memory retrieval.

Research Products

• [Journal Article] Functional roles of CREB as a positive regulator in the formation and enhancement of memory (2014)
  • Author(s): Kida, S. & Serita, T
  • Journal Title: Brain Research Bulletin Volume: (in press)
  • DOI: 10.1016/j.brainresbull.2014.04.011
  • Peer Reviewed
• [Journal Article] Interactions between αCaMKII and calmodulin in living cells : conformational changes arising from CaM -dependent and -independent relationships (2013)
  • Author(s): Kato, K., Iwamoto, T . & Kida, S
  • Journal Title: Mol. Brain Volume: 6 Pages: 37
  • DOI: 10.1186/1756-6606-6-37
  • Peer Reviewed
• [Journal Article] 記憶形成とアップデートのメカニズム (2013)
  • Author(s): 喜田聡
  • Journal Title: 化学と生物 Volume: 51 Pages: 81-89
  • Peer Reviewed
• [Journal Article] レチノイン酸受容体 (2013)
  • Author(s): 喜田聡
  • Journal Title: 分子精神医学 Volume: 13 Pages: 307-309
• [Journal Article] Genetic enhancement of neuropathic and inflammatory pain by forebrain upregulation of CREB-mediated transcription (2012)
  • Author(s): Descalzi G, Fukushima H, Suzuki A, Kida S, Zhuo M
  • Journal Title: Mol. Pain Volume: 8 Pages: 90
  • DOI: 10.1186/1744-8069-8-90
  • Peer Reviewed
• [Journal Article] Roles of CREB in the regulation of FMRP by group I metabotropic glutamate receptors in cingulate cortex (2012)
  • Author(s): Wang, H., Morishita, Y ., Miura, D., Naranjo, J.R., Kida, S. & Zhuo, M
  • Journal Title: Mol. Brain Volume: 5 Pages: 27
  • DOI: 10.1186/1756-6606-5-27
  • Peer Reviewed
• [Journal Article] Dysfunction of the RAR/RXR signaling pathway in the forebrain impairs hippocampal memory and synaptic plasticity (2012)
  • Author(s): Nomoto, M., T akeda, Y ., Uchida, S., Mitsuda, K., Enomoto, H., Saito, K., Choi, T ., Watabe, A.M., Kobayashi, S., Masushige, S., Manabe, T . & Kida, S
  • Journal Title: Mol. Brain Volume: 5 Pages: 8
  • DOI: 10.1186/1756-6606-5-8
  • Peer Reviewed
• [Journal Article] Hippocampal function is not required for the precision of remote place memory (2012)
  • Author(s): Kitamura, T ., Okubo-Suzuki, R., T akashima, N., Murayama, A., Hino, T ., Nishizono, H., Kida, S. & Inokuchi, K
  • Journal Title: Mol. Brain Volume: 5 Pages: 5
  • DOI: 10.1186/1756-6606-5-5
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of mouse TREM-1 gene in RA W264.7 macrophage-like cells (2011)
  • Author(s): Hosoda, H., T amura, H., Kida, S. & Nagaoka, I
  • Journal Title: Life Sci Volume: 89 Pages: 115-122
  • DOI: 10.1016/j.lfs.2011.05.007
  • Peer Reviewed
• [Journal Article] Up-regulation of CREB-mediated transcription enhances both short- and long-term memory (2011)
  • Author(s): Suzuki, A., Fukushima, H., T akuya Mukawa, T ., Toyoda, H., Wu, L-J., Zhao, M-G ., Hui Xu, H., Shang, Y ., Endoh, K., Iwamoto, Mamiya, N., Okano, E., Hasegawa, H., Mercaldo, V ., Y ue Zhang, Y ., Maeda, R., Ohta, M., Josselyn, S.A., Zhuo, M. & Kida, S
  • Journal Title: J . Neurosci Volume: 31 Pages: 8786-8802
  • DOI: 10.1523/JNEUROSCI.3257-10.2011
  • Peer Reviewed
• [Journal Article] Development of tightly regulated tetracycline-dependent transcriptional activator and repressor co-expression system for the strong induction of transgene expression (2011)
  • Author(s): Hosoda, H., Miyao, T ., Uchida, S., Sakai, S. & Kida, S
  • Journal Title: Cytotechnology Volume: 63 Pages: 211-216
  • DOI: 10.1007/s10616-011-9335-z
  • Peer Reviewed
• [Journal Article] 想起後の記憶制御のダイナミズム (2011)
  • Author(s): 喜田聡
  • Journal Title: 細胞工学 Volume: 30 Pages: 475-481
• [Journal Article] 核内受容体による学習記憶制御 (2011)
  • Author(s): 喜田聡
  • Journal Title: 生体の化学 Volume: 62 Pages: 486-488
• [Journal Article] Enhancement of fear memory by retrieval through reconsolidation
  • Author(s): Fukushima, H., Zhang, Y ., Archbol, G ., Ishikawa, R., Nader, K. Kida, S
  • Journal Title: eLife Volume: (in press)
  • Peer Reviewed
• [Journal Article] Time-dependent enhancement of hippocampus-dependent memory after treatment with memantine : implications for enhanced hippocampal adult neurogenesis
  • Author(s): Ishikawa, R., Kim, R., Namba, T ., Kohsaka, S., Uchino, S. & Kida, S
  • Journal Title: Hippocampus Volume: (in press)
  • DOI: 10.1002/hipo.22270
  • Peer Reviewed
• [Presentation] 記憶制御に対するビタミン A の役割 (2014)
  • Author(s): 喜田聡
  • Organizer: 第7回 レドックス・ライフイノベーション シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20140306-07
• [Presentation] カルシニューリンを起点とした想起後の受動回避記憶の増強 (2013)
  • Author(s): 福島穂高, 張 悦, 金 亮, 喜田聡
  • Organizer: 平成25年度「包括脳ネットワーク」夏のワークショップ
  • Place of Presentation: 名古屋
  • Year and Date: 20130829-0901
• [Presentation] Role of Calcineurin in Enhancement of Fear Memory after Retrieval (2013)
  • Author(s): Kida, S
  • Organizer: The 8th International Conference for Neurons and Brain Diseases
  • Place of Presentation: National University of Singapore, Singapore
  • Year and Date: 20130802-04
• [Presentation] リン酸化CREB と Erk の発現レベルを指標とした恐怖記憶強化と消去制御に関わるニューロン集団の同定 (2013)
  • Author(s): 張 悦, 福島穂高, 喜田聡
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
  • Year and Date: 20130620-23
• [Presentation] Dynamic regulation of fear memory after retrieval (2013)
  • Author(s): Kida, S
  • Organizer: Meeting México-Japan (Memory Traces & Tags)
  • Place of Presentation: Centro Académico Cultural UNAMx, Mexico
  • Year and Date: 20130425-26
• [Presentation] Enhancement of fear memory after retrieval (2013)
  • Author(s): Kida, S
  • Organizer: ISN-ASN 2013 (Mechanisms of memory enhancement of erasure)
  • Place of Presentation: Cancun, Mexico
  • Year and Date: 20130420-24
• [Presentation] Dynamic regulation of fear memory after retrieval : Therapeutic targets for the treatment of PTSD (2013)
  • Author(s): Kida, S
  • Organizer: WFSBP‐ Neuro Joint Symposium ("Neurocircuit for physiological and pathophysiological brain")
  • Place of Presentation: Kyoto
  • Year and Date: 2013-06-30
• [Presentation] Neural circuits and cellular and molecular mechanisms underlying fear learning (2013)
  • Author(s): Kida, S
  • Organizer: 11th World Congress of Biological Psychiatry (" Perspectives in neurobiology of PTSD and disaster-related psychiatric disorders")
  • Place of Presentation: Kyoto
  • Year and Date: 2013-06-25
• [Presentation] Enhancement of fear memory after retrieval (2013)
  • Author(s): Kida, S
  • Organizer: The 6th Molecular Cellular Cognition Society (MCCS)-Asia Symposium ("Molecular, Cellular and Circuit Mechanisms underlying Cognition")
  • Place of Presentation: Kyoto
  • Year and Date: 2013-06-19
• [Presentation] Distinct time-dependent roles of Erk activation in the reconsolidation and extinction memory phases (2013)
  • Author(s): Yue ZHANG , Hotaka FUKUSHIMA, Satoshi KIDA
  • Organizer: The 6th Molecular Cellular Cognition Society (MCCS)-Asia Conference
  • Place of Presentation: 京都
  • Year and Date: 2013-06-19
• [Presentation] 想起後の恐怖記憶制御のダイナミクス (2013)
  • Author(s): 喜田聡
  • Organizer: 自然科学研究機構国際拠点形成プロジェクト「脳の階層的研究」報告会&シンポジウム
  • Place of Presentation: 岡崎
  • Year and Date: 2013-03-05
• [Presentation] Dynamic regulation of fear memory after retrieval (2013)
  • Author(s): Kida S
  • Organizer: MCCS-Australia (Asia-pacific)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2013-02-02
• [Presentation] タンパク質分解を起点とした想起後の記憶制御 (2012)
  • Author(s): 喜田聡
  • Organizer: 生理学研究所シンポジウム「記憶回路研究会」個体内記憶回路の同定とその機能解析による学習記憶制御基盤の統合的理解
  • Place of Presentation: 岡崎
  • Year and Date: 20121120-21
• [Presentation] Distinct neuron populations in the enhancement/reconsolidation and extinction phases of reactivated inhibitory avoidance memory in the hippocampus, amygdala and mPFC (2012)
  • Author(s): Yue ZHANG , Hotaka FUKUSHIMA , Satoshi KIDA
  • Organizer: The 42nd annual meeting of Society for neuroscience
  • Place of Presentation: New Orleans
  • Year and Date: 20121013-17
• [Presentation] CREB と ERKのリン酸化を指標とした恐怖記憶強化と消去制御に関わるニューロン集団の同定 (2012)
  • Author(s): 張 悦, 福島穂高, 喜田聡
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] Dynamic regulation of memory after retrieval (2012)
  • Author(s): 喜田聡
  • Organizer: 1st frontier international symposium for neurons and disease
  • Place of Presentation: Xian, China
  • Year and Date: 20120322-25
• [Presentation] Enhancement of fear memory after retrieval through protein degradation and synthesis (2012)
  • Author(s): 喜田聡
  • Organizer: 第35回日本分子生物学会ワークショップ 「遺伝子発現とタンパク質分解によって産み出される記憶制御のダイナミクス」
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] 特異的ニューロン集団が産み出す想起後の記憶制御のダイナミクス (2012)
  • Author(s): 喜田聡
  • Organizer: 第35回日本神経科学大会シンポジウム「個々の神経細胞の働きを俯瞰して見る脳機能」
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-19
• [Presentation] Dynamic regulation of fear memory after retrieval by distinct neural circuits (2012)
  • Author(s): 喜 田 聡
  • Organizer: National Institute for Physiological Sciences International Workshop 2012 Central Neuroplasticity in Sensory-Emotional Link
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2012-09-15
• [Presentation] Circadian regulation of memory retrieval by BMAL1 (2012)
  • Author(s): 喜田聡
  • Organizer: EMCCS--FENS 5th annual meeting
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2012-07-13
• [Presentation] Dynamic regulation of fear memory after retrieval (2012)
  • Author(s): 喜田聡
  • Organizer: Bio-X 2012 International Symposium on Molecular Cognition and Translational Research on Neuropsychiatric Disorders
  • Place of Presentation: Shanghai, China
  • Year and Date: 2012-04-29
• [Presentation] 恐怖記憶制御のダイナミクス,第153回日本獣医学会学術集会シンポジウム (2012)
  • Author(s): 喜田聡
  • Organizer: 本能・情動行動の統合・調節メカニズム「神経生理学研究の挑戦 : 動物の情動行動の謎を解く」
  • Place of Presentation: 大宮
  • Year and Date: 2012-03-27
• [Presentation] CREB と Erkの活性化を指標とした恐怖記憶強化及び消去を制御するニューロン集団の同定 (2012)
  • Author(s): 張 悦, 福島穂高, 喜田聡
  • Organizer: 第56回日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] CREB 情報伝達系活性化が学習能力に与える影響の解析 (2012)
  • Author(s): 芹田龍郎, 福島穂高, 喜田聡
  • Organizer: 第56回日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] タンパク質合成と分解を介した想起後の受動回避記憶の強化 (2011)
  • Author(s): 福島穂高, 張悦, 喜田聡
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] CREB と Erkのリン酸化を指標とした恐怖記憶再固定化及び消去制御に関わるニューロン集団の同定 (2011)
  • Author(s): 張 悦, 福島穂高, 喜田聡
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Mechanisms of enhancement and reconsolidation of reactivated inhibitory avoidance memory (2011)
  • Author(s): Hotaka FUKUSHIMA, Yue ZHANG , Satoshi KIDA
  • Organizer: Naito Conference on "Biological Basis of Mental Functions and Disorder"
  • Place of Presentation: 山梨
  • Year and Date: 2011-10-18
• [Presentation] 想起後の記憶制御のダイナミズム (2011)
  • Author(s): 喜田 聡, 福島穂高, 張悦
  • Organizer: 日本神経科学会シンポジウム「記憶形成のダイナミクス」
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] 想起後の受動回避記憶強化誘導時の脳領野間相互作用機構の解析 (2011)
  • Author(s): 福島穂高, 張悦, 喜田聡
  • Organizer: 日本神経科学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] 免疫染色法を用いた受動的回避記憶の固定化に関与する脳領域の同定及び役割の解析 (2011)
  • Author(s): 張 悦, 福島穂高, 喜田聡
  • Organizer: 日本神経科学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-16
• [Presentation] Distinct regulations of reconsolidation and extinction phases of fear memory at the behavioral and molecular levels (2011)
  • Author(s): 喜田聡
  • Organizer: International Summer Conference of Neurons and Brain Diseases
  • Place of Presentation: 富山
  • Year and Date: 2011-08-04
• [Presentation] Enhancement of fear memory after retrieval (2011)
  • Author(s): 喜田聡
  • Organizer: Fifth Annual Canadian Neuroscience Meeting
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2011-06-01
• [Book] 精神疾患のマイクロエンドフェノタイプ, メンタル医療(糸川昌成監修) (2013)
  • Author(s): 喜田 聡
  • Total Pages: 221(99-105)
  • Publisher: シーエムシー出版
• [Book] Memory reconsolidation versus extinction,「 Memory reconsolidation 」 (Cristina Alberini 編集) (2013)
  • Author(s): Kida, S
  • Total Pages: 336(119-137)
  • Publisher: Elsevier 社
• [Book] Memory reconsolidation and extinction, 「Memory Mechanisms on Health and Disease」(Karl Peter Giese 編集) (2012)
  • Author(s): 喜田 聡
  • Total Pages: 444(20)
  • Publisher: World Scientific 社
• [Book] 編集委員・三品昌美, 「CREB 変異マウス」モデル動物利用マニュアル 「疾患モデルの作製と利用 ; 脳 ・ 神経疾患 (2011)
  • Author(s): 喜田 聡
  • Total Pages: 310
  • Publisher: 株式会社エル ・ アイ ・ シー