2013 Fiscal Year Final Research Report
Establishment of evolutional microscopy on neurons with ultra-fast two-photon microscope
| Project/Area Number |
23300121
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Neuroscience in general
|
|---|
| Research Institution | Waseda University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 分子動態 / タンパク質 / 神経細胞 / シナプス / 活動電位 / 膜電位計測 |
|---|
| Research Abstract |
This research project aimed to measure dynamics of protein molecules rapidly moving in the cytosol of neuronal dendrites at multiple points simultaneously by means of a newly developed two-photon microscope. I succeeded in recording at multiple points at more than 10 kHz of time resolution from cytosol of primary cultured neurons derived from mouse, in which GFP or GFP-fused CamKII proteins were expressed, and in analyzing by FCS. I was also successful in recording action potentials from multiple cultured neurons, which enabled to analyze synaptic connections between neuron pairs and also direction of synaptic connections.
|
Research Products
(13 results)
