2013 Fiscal Year Final Research Report
Analysis of the mechanism of neurite outgrowth driven by membrane transport and cytoskeletal reorganization
| Project/Area Number |
23300134
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 神経突起伸展 / 小胞輸送 / FRET / Rab / Rho |
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| Research Abstract |
Cytoskeletal reorganization and the membrane addition are pivotal for neurite outgrowth. Here we studied the regulation-mechanism of membrane addition using FRET biosensors. TC10 activity at the plasma membrane decreased at extending growth cones. GTP hydrolysis of TC10 promotes neurite outgrowth through exocytic fusion of Rab11- and L1-containing vesicles by releasing exocyst component Exo70. We developed FRET sensor for Rab35, which promotes neurite outgrowth. Using Rab35 sensor, we showed that Rab35 activity at plasma membrane of growth cones was higher than that of neurite shafts and cell bodies. Phosphorylation of STEF by PKA is critical for Rac1 activation and neurite outgrowth in dbcAMP-treated PC12D cells. We propose that local activation of Rac1 and Cdc42 at neurite tips is necessary for neurite outgrowth. Longest neurite-specific activation of Rap1B in hippocampal neurons contributes to polarity formation through RalA and Nore1A in addition to PI3-kinase.
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[Presentation] A role of the Rac1-TC10 axis in neurite outgrowth2012
Author(s)
Fujita, A., Koinuma, S., Yasuda, S, Nagai, H., Wada, N., Nakamura, T
Organizer
2012 ASCB annual meeting
Place of Presentation
San Francisco, USA
Year and Date
20121211-14
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[Presentation] Analysis of Rab35 function in neurite outgrowth using FRET biosensors2012
Author(s)
Ishido, N., Nagai, H., Kobayashi, H., Arai, T., Fukuda, M., and Nakamura, T
Organizer
SfN2012
Place of Presentation
New Orleans, USA
Year and Date
20121013-17
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[Presentation] The Rho family GTPase TC10 is implicated in neurite outgrowth through membrane addition2012
Author(s)
Fujita, A., Koinuma, S., Nagai, H., Yasuda, S., Wada, N., and Nakamura, T
Organizer
第56回神経化学会大会
Place of Presentation
神戸
Year and Date
20120900
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[Presentation] Analysis of rab35 function in neurite outgrowth using FRET biosensors2011
Author(s)
Ishido, N.., Nagai, H., Kobayashi, H., Sako, Y., Arai, T., Hattori, S., Matsuda, M., Fukuda, M., and Nakamura, T
Organizer
第34回日本分子生物学会年会
Place of Presentation
横浜
Year and Date
20111200
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[Presentation] A novel mode of neuritogenesis regulation : a spatiotemporal change of Rab35 activity during nerve growth factor-induced neurite outgrowth2011
Author(s)
Nakamura, T., Ishido, N, Obata,Y., Kobayashi, H., Yasuda, S., Koizumi, H., Arai, T., Fukuda, M., and Matsuda, M
Organizer
The 30th NAITO Conference on Membrane Dynamics and Lipid Biology (II)
Place of Presentation
Sapporo
Year and Date
20110600
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[Presentation] Develpoment of genetically-encoded FRET biosensors for Rab352011
Author(s)
Ishido, N.., Kobayashi, H., Sako, Y., Arai, T., Hattori, S., Matsuda, M., Fukuda, M., and Nakamura, T
Organizer
第63回日本細胞生物学会大会
Place of Presentation
札幌
Year and Date
20110600
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