2013 Fiscal Year Final Research Report

Immunological function and infection control analysis of murine norovirus capsid protein P domain

Research Project

Project/Area Number	23300153
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Laboratory animal science
Research Institution	Jichi Medical University
Principal Investigator	KUNITA SATOSHI 自治医科大学, 医学部, 教授 (10195472)
Co-Investigator(Kenkyū-buntansha)	YAGAMI Ken-ichi 筑波大学, 医学医療系, 教授 (40166476)
Project Period (FY)	2011-04-01 – 2014-03-31
Keywords	マウスノロウイルス / キャプシドタンパク質 / 診断 / 抗体 / 抗原性 / 免疫原性 / 感染阻止
Research Abstract	Antibody measurement methods for murine norovirus (MNV) with high specificity and sensitivity were established using the recombinant VP1 protein of MNV. We found that mice can be persistently infected with MNV. The infected mice served as a source of infection for more than one year, and high blood antibody was maintained. Examination of strongly immunogenic region within VP1 protein revealed that VP1-P2 subdomain has the most potent antibody-induced activity. However, a simple approach to induce systemic antibodies to VP1-P2 subdomain could not prevent MNV infection in mice. It may be necessary to induce intestinal immunity such as mucosal IgA to control MNV infection effectively.

(3 results)