Development of tools for analysis of role of D-amino acids using molecularly imprinted polymer

2014 Fiscal Year Final Research Report

• Project/Area Number: 23300180
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Shibaura Institute of Technology
• Principal Investigator: YOSHIMI Yasuo 芝浦工業大学, 工学部, 教授 (30267421)
• Co-Investigator(Kenkyū-buntansha): 長濱 辰文 東邦大学, 薬学部, 教授 (70145001)
• Co-Investigator(Renkei-kenkyūsha): 丹羽 修 産業技術総合研究所, バイオメディカル研究部門, 主幹研究員 (70392644)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: Molecular imprinting / sensor / imaging / chrality / nano particles / D-amino acids / nueron / Aplysia

Outline of Final Research Achievements

Development of chiral specific sensor is needed for analysis of the role of D-amino acids in central nervous system, however chiral specificity of an electrode grafted with molecularly imprinted polymer (MIP) is declined in aqueous system. During the supported term, chiral specific sensing with MIP of an amino acid can be performed by blending hydrophilic and hydrophobic crosslinking monomers.
Voltage sensitive dye (VSD) imaging technique is needed to analyze dependency of neural network on D-amino acids. However VSD imaging is yet to be established for Aplysia central nervous system. We developed new VSD imaging method by using di-4-ANEPPS and tetraethyl ammonium cation. The method successfully detect action potential responding to taste stimulation on the mouth of Aplysia.
Those established method is feasible for analysis of the role of D-amino acids in central nervous system which is suspected to be important factor of nervous deceases.

Free Research Field

医用化学工学