2013 Fiscal Year Final Research Report
Super-selective molecular delivery system of metastatic lymph node using nanobubbles and ultrasound
| Project/Area Number |
23300183
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJIKAWA Shigeo 北海道大学, 大学院・工学研究科, 名誉教授 (70111937)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | リンパ節転移 / ナノバブル / 超音波 / 分子導入 |
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| Research Abstract |
The purpose of this study was to develop a super-selective molecular delivery system of lymph node metastasis via lymphatic vessels using nanobubbles (NBs) and ultrasound (US). We used MXH10/Mo-lpr/lpr mice exhibiting remarkable systemic lymphadenopathy, with proper axillary lymph nodes (PALNs) and subiliac lymph nodes (SiLNs) that are 6 to 12 mm in diameter. First, we identified 22 different LNs situated in the head and neck, limbs, thoracic and abdominal regions. The metastasis route was found to be via the efferent lymphatic vessels of the SiLN, and metastasis incidence depended on the number of cells injected, the injection duration and the SiLN volume. Next, we investigated drug delivery efficiency to the lymph node metastasis by the molecular delivery method using NBs and US. It was found that exposure to US in the presence of NBs enhanced the delivery of fluorophores into the lymphocytes near the lymphatic channels, compared to the use of US in the absence of NBs.
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[Presentation] Flow characteristics of the lymphatic flow to the axillary lymph node driven by increasing pressures in the inguinal lymph node2012
Author(s)
Hatakeyama Y, Sax N, Li L, Kato S, Okuno T, Miura Y, Shao L, Sakamoto M, Mori S, Fujikawa S , Kodama T
Organizer
Medical Physics and Biomedical Engineering
Place of Presentation
International Convention Center, Beijing, China
Year and Date
20120526-31
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[Presentation] Effects of the liposomal formuilation on the behavior and physical characteristics of acoustic liposomes2012
Author(s)
Sax N, Horie S, Li L, Li S, Kochi T, Chen R, Watanabe Y, Yagishita Y, Sakamoto M, Mori S, Kodama T
Organizer
The 19th International Symposium on Nonliner Acoustics
Place of Presentation
International Conference Center ofWaseda University
Year and Date
20120521-24
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