Ultrasound cancer therapy in next-generation with liposomal nanobubbles having both functions of diagnostics and therapeutics

2013 Fiscal Year Final Research Report

• Project/Area Number: 23300192
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Teikyo University
• Principal Investigator: MARUYAMA Kazuo 帝京大学, 薬学部, 教授 (30130040)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Ryo 帝京大学, 薬学部, 准教授 (90384784) ; ODA Yusuke 帝京大学, 薬学部, 助手 (80505941) ; TAKAHASHI Hideyo 帝京大学, 薬学部, 教授 (10266348)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 低侵襲治療システム / 超音波医科学

Research Abstract

F-DPPC for high PFC was newly synthesized as shell of BL. Fluorinated phospholipid (F-DPPC) was synthesized as a new outer shell material of BL for the purpose of retention of PFC. RGD-BL (DSPC:F-DPPC:DSPE-PEG2000:DSPE-PEG3000-Mal=44:50:5:1 (molar ratio)) encapsulating mixed gas of C5F12:C6F14=1:1 (weight ratio), was prepared with about 500 nm in diameter. The combination of RGD-BL and diagnostic US make the image of the thrombus and neovasculature of solid tumor. The combination of RGD-BL and therapeutic US can be use for gene therapy by introduction of pCMV-IL12 and hyperthermia treatment with intratumoral temperature rise. Thus, the combination of RGD-BL and US may be used for ultrasound theranostics system with therapeutic and diagnostic, which is a next-generation.

Research Products

• [Journal Article] Anti-tumor effect of NF-ĸB decoy transfer by mannose-modified bubble lipoplex into macrophages in mouse malignant ascites (2014)
  • Author(s): Kono Yusuke, Kawakami Shigeru, Higuchi Yuriko, Maruyama Kazuo, Yamashita Fumiyoshi, Hashida Mitsuru
  • Journal Title: Cancer Science Volume: (in press)
  • Peer Reviewed
• [Journal Article] Evaluation of active control of Bubble liposomes in a bifurcated flow under various ultrasound conditions (2014)
  • Author(s): Ren Koda, Jun Koido, Naoto Hosaka, Shinya Onogi, Takashi Mochizuki, Kohji Masuda, Ryo Suzuki, Kazuo Maruyama
  • Journal Title: Advanced Biomedical Engineering Volume: 3 Pages: 21-28
  • Peer Reviewed
• [Journal Article] Combination of Bubble liposomes and high-intensity focused ultrasound (HIFU) enhanced antitumor effect by tumor ablation (2014)
  • Author(s): Nobuhito Hamano, Yoichi Negishi, Kyohei Takatori, Yoko Endo-Takahashi, Ryo Suzuki, Kazuo Maruyama, Takuro Niidome, Yukihiko Aramaki
  • Journal Title: Biological & Pharmaceutical Bulletin Volume: 37(1) Pages: 174-177
  • Peer Reviewed
• [Journal Article] pDNA-loaded bubble liposomes as potential ultrasound imaging and gene delivery agents (2013)
  • Author(s): Yoko E. Takahashi, Yoichi Negishi, Arisa Nakamura, Daichi Suzuki, Saori Ukai, Katsutoshi Sugimoto, Fuminori Mariyasu, Norio Takagi, Ryo Suzuki, Kazuo Maruyama, Yukihiko Aramaki
  • Journal Title: Bioma terials Volume: 34 Pages: 2807-2813
  • Peer Reviewed
• [Journal Article] Thrombus targeted perfluorocarbon containing liposomal bubbles for enhancement of ultrasonic thrombolysis - in vitro and in vivo study - (2013)
  • Author(s): Kohsuke Hagisawa, Toshihiko Nishioka, Ryo Suzuki, Kazuo Maruyama, Yasuhiro Nishida, Kiyoshi Iida, Huai Luo, Robert J. Siegel
  • Journal Title: J. Thrombosis and Haemostasis Volume: 11 Pages: 1565-1573
  • Peer Reviewed
• [Journal Article] Prophylactic immunization with Bubble liposomes and ultrasound-treated dendritic cells provided a four-fold decrease in the frequency of melanoma lung metastasis (2012)
  • Author(s): Yusuke Oda, Ryo Suzuki, Shota Otake, Norihito Nishiie, Keiichi Hirata, Risa Koshima, Tetsuya Nomura, Naoki Utoguchi, Nobuki Kudo, Katsuro Tachibana, Kazuo Maruyama
  • Journal Title: J. Controlled Release Volume: 160 Pages: 362-366
  • Peer Reviewed
• [Presentation] 超音波によるt-PA血栓溶解加速効果の音響強度定量的解析 (2014)
  • Author(s): 澤口能一, 鈴木亮, 小田雄介, 小俣大樹, 宇留賀仁史, 関むつみ, 丸山一雄
  • Organizer: 第134回日本薬学会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-27
• [Presentation] フッ素を含有した新規リン脂質の開発 (2014)
  • Author(s): 森達也, 小田雄介, 宇留賀仁史, 鈴木亮, 丸山一雄, 忍足鉄太, 夏苅英昭, 高橋秀依
  • Organizer: 第134回日本薬学会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-27
• [Presentation] 超音波セラノスティクスシステムの開発 (2013)
  • Author(s): 丸山一雄
  • Organizer: 第16回ヒューマンサイエンス総合研究ワークショップ
  • Place of Presentation: 東京
  • Year and Date: 20131225-26
• [Presentation] Cancer gene therapy with nano-bubble associated sonoporation (2013)
  • Author(s): Ryo Suzuki
  • Organizer: ICMBU2013
  • Place of Presentation: 台湾大学
  • Year and Date: 20131022-23
• [Presentation] Development of thrombus targeting bubble liposome for diagnostic and tPA thrombolysis acceleration (2013)
  • Author(s): Yoshikazu Sawaguchi, Ryo Suzuki, Yusuke Oda, Daiki Omata, Hitoshi Uruga, Mutsumi Seki, Kohsuke Hagisawa, Kazuo Maruyama
  • Organizer: 16th World Neurosonology Meeting
  • Place of Presentation: Sofia, Bulgaria
  • Year and Date: 20131017-20
• [Presentation] The thrombus imaging by thrombus targeted bubble liposome (2013)
  • Author(s): Yoshikazu Sawaguchi, Ryo Suzuki, Yusuke Oda, Daiki Omata, Uruga Hitoshi, Mutsumi Seki, Kohsuke Hagisawa, Kazuo Maruyama
  • Organizer: 第22回日本バイオイメージング学会学術集会
  • Place of Presentation: 東京大学薬学部
  • Year and Date: 20130914-16
• [Presentation] 超音波セラノスティクス (2013)
  • Author(s): 丸山一雄
  • Organizer: 日本超音波医学会第86回学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20130524-26
  • Invited
• [Presentation] 血栓標的型バブルリポソームを用いた血栓 in vivo イメージング (2013)
  • Author(s): 澤口能一, 鈴木 亮, 小田雄介, 小俣大樹, 宇留賀仁史, 関むつみ, 萩沢康介, 丸山一雄
  • Organizer: 第57回日本薬学会関東支部会
  • Place of Presentation: 東京
  • Year and Date: 2013-10-26
• [Presentation] 超音波セラノスティックス(THERANOSTICS) (2013)
  • Author(s): 丸山一雄
  • Organizer: 第13回遺伝子・デリバリー研究会シンポジウム
  • Place of Presentation: 帝京大学
  • Year and Date: 2013-05-11
  • Invited
• [Presentation] バブルリポソーム併用血栓溶解療法の基礎検討 (2013)
  • Author(s): 澤口能一, 小田雄介, 小俣大樹, 鈴木 亮, 萩沢康介, 丸山一雄
  • Organizer: 第133回日本薬学会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-28
• [Presentation] Gene Delivery System by Cavitation of Bubble Liposome and Ultrasound in Cancer Therapy (2012)
  • Author(s): Kazuo Maruyama, Yusuke Oda, Ryo Suzuki
  • Organizer: 8th International Symposium on Cavitation
  • Place of Presentation: シンガポール
  • Year and Date: 20120814-16
• [Remarks]
  • URL: http://www.teikyo-dds-lab.com