2013 Fiscal Year Final Research Report
Development of cell internalizing antibody as a safe and nontoxic imaging tool
| Project/Area Number |
23300195
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KAUL Sunil 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 上級主任研究員 (10356751)
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| Co-Investigator(Kenkyū-buntansha) |
WADHWA Renu 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (30371090)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 検査・診断システム / バイオイメージング / 抗モータリン抗体 |
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| Research Abstract |
In order to develop application of anti-mortalin antibody for diagnostics and delivery, we generated new antibodies and performed a characterization. We found that the cell internalizing and non-internalizing antibodies possess distinctly different epitopes. Mechanism of internalization was determined by adopting multiple inhibitor approach. We found that the antibody internalization was inhibited most strongly by Nystatin, implying that it involves lipid raft mediated endocytosis. We next used specific inhibitors of three raft pathways. The internalization was strongly inhibited by dynamin inhibitor. On the other hand, caveolin-1 inhibitor and CDC42 inhibitor showed weak effect. It was concluded that the internalization of new internalizing anti-mortalin antibodies is mostly mediated by Dynamin and Caveolin dependent endocytosis.
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[Journal Article] Mortalin antibody-conjugated quantum dot transfer from human mesenchymal stromal cells to breast cancer cells requires cell-cell interaction2013
Author(s)
Pietilä, M., Lehenkari, P., Kuvaja, P., M., Kaul, S. C., Wadhwa, R., and Uemura T
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Journal Title
Exp. Cell Res
Volume: 319
Pages: 2770-2780
DOI
Peer Reviewed
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[Journal Article] Molecular interactions of Bcl-2 and Bcl-xL with mortalin : Identification and functional characterization2013
Author(s)
Saxena, N., Katiyar, SP., Liu, Y., Grover, A., Sundar, D., Kaul, S. C., and Wadhwa, R
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Journal Title
Biosci. Rep
Volume: 33
Pages: 797-806
DOI
Peer Reviewed
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[Journal Article] Withanone binds to mortalin and abrogates mortalin-p53 complex : Computational and experimental evidence2012
Author(s)
Grover, A., Priyandoko, D., Gao, R., Shandilya, A., Widodo, N., Bisaria, V. S., Kaul, S. C., Wadhwa, R, and Sundar, D
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Journal Title
Int. J. Biochem. Cell Biol
Volume: 44
Pages: 496-504
DOI
Peer Reviewed
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[Presentation] Cell internalizing anti-mortalin antibodies for in vitro and in vivo imaging applications : mechanism2014
Author(s)
Wadhwa, R., Kaul, Z., Na, Y., Ryu, J., Yun, C-O., and Kaul, S
Organizer
日本組織培養学会第87回大会
Place of Presentation
星陵会館(東京都)
Year and Date
2014-05-29
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[Presentation] Fate of bone marrow mesenchymal stem cells following the autologous transplantation into a rabbit osteonecrosis model2013
Author(s)
Sugaya, H., Mishima, H., Li, M., Aoto, K., Yoshioka, T., Ogawa, T., Yaguchi, T., Gao, R., Kaul, S., and Wadhwa, R
Organizer
日本組織培養学会第86回大会
Place of Presentation
産業技術総合研究所(茨城県)
Year and Date
2013-05-30
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[Presentation] Long term labeling of mesenchymal stem cells by internalizing quantum dots conjugated with anti-mortalin antibody : application to cartilage regeneration and study of cancer cells/stem cells interaction2013
Author(s)
Uemura, T., Yoshioka, T., Pietilae, M., Mishima, H., Long, X., Matsumoto, R., Kaul, S., and Wadhwa, R
Organizer
日本組織培養学会第86回大会
Place of Presentation
産業技術総合研究所(茨城県)
Year and Date
2013-05-30
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[Presentation] Non-mitochondrial mortalin promotes human tumorigenesis through activation of telomerase and hnRNP-K2013
Author(s)
Ryu, J., Yaguchi, T., Na, Y., Yun, Ch., Kaul, S.C. and Wadhwa, R
Organizer
日本組織培養学会第86回大会
Place of Presentation
産業技術総合研究所(茨城県)
Year and Date
2013-05-30
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