2013 Fiscal Year Final Research Report
Development of single chain antibodies against sugar chain tumor antigen on the surface of leukemia cells and their application for order-made medicine
| Project/Area Number |
23300356
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor diagnosis
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| Research Institution | Kagoshima University |
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Principal Investigator |
SUDA Yasuo 鹿児島大学, 理工学研究科, 教授 (70179282)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Yuji 鹿児島大学, 理工学研究科, 教授 (60223195)
ARIMA Naomichi 鹿児島大学, 医歯(薬)学総合研究科, 教授 (30175997)
WAKAO Masahiro 鹿児島大学, 理工学研究科, 助教 (20404535)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 腫瘍マーカー / オーダーメード治療 |
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| Research Abstract |
Specifically expressed sugar chains on adult T-cell leukemia(ATL) cells should be tumor markers,which can be appropriate antigen molecules in the targeted therapy using ant ibodies.Trace amount of sugar chains extracted and fractionated from tumor ATL cells were immobilized on the top of the optical fiber(50) chemically modified with gold nanoparticles to prepare fiber-type sugar chip. Using the chip and localized surface Plasmon resonance method,the screening of phage display was performed to obtain phages expressing single chain antibody(scFV)which bound to sugar chain from ATL cells. then,severel scFv were isolated from the selected phages.One scFv was then immobilized to fluorescent nanoparticles(FNP) with a optimal conditions to prepare scFv-FNP. By the FACS analysis and fluorescent microscopy,the scFv-FNP was found to bind specifically to cultured ATL cell(S1T),but not to non ATL leukemia cells(CEM),indicating the easy detection tool for ATL cells.
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Research Products
(35 results)
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[Journal Article] Super high sensitive detection of viruses using sugar-chain immobilized gold nanoparticles (SGNPs)2012
Author(s)
Zang X, Nakamura-Tsuruta S, Haruyama M, Yokoyama R, Nagatomo M, Wakao M, Nakajima K, Aoyama K, Okuno T, Morikawa S, Hiroi S, Kase T, Nose H, Nishi J, Okamoto M, Baba M, Suda Y
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Journal Title
Po1ymer Preprints
Volume: 53
Pages: 671-672
Peer Reviewed
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[Presentation] 抗体治療を目的とした成人T細胞白血病(ATL)細胞表層の糖鎖に結合する一本鎖抗体(scFV)の開発2012
Author(s)
佐藤綾香, 田添安里紗, 中森百恵, 木地山真実, 前原千恵里, 岩切健二, 若尾雅広, 伊東祐二, 有馬直道, 隅田泰生
Organizer
第31回日本糖質学会学年会
Place of Presentation
(ポスター発表P‐151), 鹿児島市民文化ホール
Year and Date
20120912-20
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[Presentation] Super high senstive detection of viruses using sugar-chain immobilized gold nanoparticles(SGNPs)2012
Author(s)
Zang X, Nakamura-Tsuruta S, Haruyama M, Yokoyama R, Nagatomo M, Wakao M, Nakajima K, Aoyama K, Okuno T, Morikawa S, Hiroi S, Kase T, Nose H, Nishi J, Okamoto M, Baba M, Suda Y
Organizer
243rd American Chemical Society National Meeting & Exposition
Place of Presentation
San Diego, California
Year and Date
2012-03-27
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[Presentation] 成人T細胞白血病(ATL)細胞表層の糖鎖に結合する一本鎖抗体(scFv)の開発2011
Author(s)
佐藤綾香, 岩切健二, 仮屋博敬, 木地山真実, 前原千恵里, 岡田摩耶, 戎義子, 若尾雅広, 有馬直道, 伊東祐二, 隅田泰生
Organizer
第84回日本生化学会大会
Place of Presentation
国立京都国際会館, 京都府京都市
Year and Date
20110921-24
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