Development of new assay for evaluating chronic effects of eutrophication in coral reefs

2013 Fiscal Year Final Research Report

• Project/Area Number: 23310025
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental impact assessment/Environmental policy
• Research Institution: University of the Ryukyus
• Principal Investigator: YAMASAKI Hideo 琉球大学, 理学部, 教授 (40222369)
• Co-Investigator(Kenkyū-buntansha): SUDA Shoichiro 琉球大学, 理学部, 教授 (00359986)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: サンゴ礁 / 富栄養化 / 活性窒素 / ストレス / 慢性毒性

Research Abstract

Eutrophication of seawater caused by run-off from land appears to change the reef ecosystems in Okinawa. Until now, however, there has been no measure to assess its physiological impact on reef-building corals. A major objective of this research project was to find a quantitative measure for the physiological impact of eutrophication on a biochemical basis. Focusing on uncontrolled production of reactive nitrogen species (RNS) under stress conditions, this study attempted to quantify nitrated biomolecules with simple procedures. We suggest that hydroxyl radical generated by the Fenton reaction liberates nitro group from its parent compounds and produces nitrate ion that can be quantified with ion chromatography technique, a novel method to evaluate nitrogen toxicity.

Research Products

• [Journal Article] 硝酸塩・亜硝酸塩を基質としたNO合成機構研究の現在・過去・未来 (2014)
  • Author(s): 山崎秀雄・渡邊なお子
  • Journal Title: 日本心脈管作動物質学会機 関誌 Japanese Society for Circulation Research 「血管」 Volume: vol. 37
  • Peer Reviewed
• [Journal Article] Nitrite-dependent nitric oxide production pathway : diversity of NO production systems (2014)
  • Author(s): Yamasaki, H., Watanabe, N. S., Fukuto, J. and Cohen, M. F.
  • Journal Title: In Oxidative stress in applied basic research and clinical practice : studies on pediatric disorders (Tsukahara H, et al. eds)
  • Peer Reviewed
• [Journal Article] Controlled free radical attack in the apoplast : A hypothesis for roles of O, N and S species in regulatory and polysaccharide cleavage events during rapid abscission by Azolla (2014)
  • Author(s): Cohen, M. F., Gurung, S., Fukuto, J. and Yamasaki, H.
  • Journal Title: Plant Science Volume: 217 Pages: 120-126
  • Peer Reviewed
• [Journal Article] Azide-dependent NO emission from the water fern Azolla pinnata (2014)
  • Author(s): Gurung, S., Cohen, M. F. and Yamasaki, H.
  • Journal Title: Russian Journal of Plant Physiology Volume: 61 Pages: 543-547
  • Peer Reviewed
• [Journal Article] Kurahyne, an acetylene-containing lipopeptide from a marine cyanobacterial assemblage of Lyngbya sp (2014)
  • Author(s): Iwasaki, A., Ohno, O., Sumimoto, S., Suda, S. and Suenaga, K.
  • Journal Title: RSC Advances Volume: 4 Pages: 12840-12845
  • Peer Reviewed
• [Journal Article] Kurahamide, a cyclic depsipeptide analog of Dolastatin 13 from a marine cyanobacterial assemblage Lyngbya sp (2014)
  • Author(s): Iwasaki, A., Sumimoto, S., Ohno, O., Suda, S. and Suenaga, K.
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 87 Pages: 609-613
  • Peer Reviewed
• [Journal Article] Genetic diversity of filamentous cyanobacteria from shore regions of Okinawa (2013)
  • Author(s): Suda, S., Moriya, R., Sumimoto, S., Ohno, O. and Suenaga, K.
  • Journal Title: Journal of Marine Science and Technology TAIWAN 21 Pages: 175-180
  • Peer Reviewed
• [Journal Article] Polyamine-induced rapid root abscission in Azolla pinnata (2012)
  • Author(s): Gurung, S., Cohen, M. F., Fukuto, J. and Yamasaki, H.
  • Journal Title: Journal of Amino Acids Volume: Volume 2012, (Article ID 493209) Pages: 9
  • DOI: 10.1155/2012/493209
  • Peer Reviewed
• [Journal Article] Phenotypic variations in croton Codiaeum variegatum (L.) Blume characterized by digital image-based procedure (2012)
  • Author(s): Mollick, A. S. and Yamasaki, H.
  • Journal Title: Acta Horticulturae Volume: 937 Pages: 393-400
  • Peer Reviewed
• [Journal Article] Ontogenetic change in the lipid and fatty acid composition of scleractinian coral larvae (2012)
  • Author(s): Figueiredo, J., Baird, A. H., Cohen, M. F., Flot, J.-F., Kamiki, T., Meziane, T., Tsuchiya, M. and Yamasaki, H.
  • Journal Title: Coral Reefs Volume: 31 Pages: 613-619
  • Peer Reviewed
• [Journal Article] Croton Codiaeum variegatum (L.) Blume cultivars characterized by leaf phenotypic parameters (2011)
  • Author(s): Mollick, A. S., Shimojia, H., Denda, T., Yokota, M. and Yamasaki, H.
  • Journal Title: Scientia Horticulturae Volume: 132 Pages: 71-79
  • Peer Reviewed
• [Journal Article] Heat-shock-induced color-pattern changes of the blue pansy butterfly Junonia orithya : Physiological and evolutionary implications (2011)
  • Author(s): Mahdi, S. H. A., Yamasaki, H., and Otaki, J. M.
  • Journal Title: Journal of Thermal Biology Volume: 36 Pages: 312-321
  • Peer Reviewed
• [Journal Article] Effect of nitric oxide on leaf non photochemical quenching under heat-stress conditions (2011)
  • Author(s): Hossain, K. K., Nakamura, T. and Yamasaki, H.
  • Journal Title: Russian Journal of Plant Physiology Volume: 58 Pages: 629-633
  • Peer Reviewed
• [Journal Article] Nitric oxide synthase-like activities in plants (2011)
  • Author(s): Yamasaki, H., Itoh, R.D., Bouchard, J.N., Dghim, A.A., Hossain, K.K., Gurung, S., Cohen, M.F.
  • Journal Title: Nitrogen Metabolism plants in the Post-Genomic Era (Foyer, C.H. and Zhang H. eds) Volume: Volume 42 Pages: 103-125
  • Peer Reviewed
• [Presentation] サンゴ白化現象における一酸化窒素NOの関与 : 活性窒素障害評価法の探索 (2013)
  • Author(s): 山崎秀雄・渡邊なお子
  • Organizer: 日本サンゴ礁学会第16回大会
  • Place of Presentation: 沖縄
  • Year and Date: 20131200
• [Presentation] Azide-dependent NO emission from the water fern Azolla pinata (2013)
  • Author(s): Gurung, S., Cohen, M. F. and Yamasaki, H.
  • Organizer: 第13回日本NO学会学術集会
  • Place of Presentation: 沖縄
  • Year and Date: 20130600
• [Presentation] 沖縄食材の亜硝酸供与ポテンシャル (2013)
  • Author(s): 渡邊なお子・山崎秀雄
  • Organizer: 第13回日本NO学会学術集会
  • Place of Presentation: 沖縄
  • Year and Date: 20130600
• [Presentation] Genetic diversity of filamentous cyanobacteria from shore regions of Okinawa (2013)
  • Author(s): Sumimoto, S., Moriya, R. and Suda, S.
  • Organizer: 9th East China Sea Conference, Keelung
  • Place of Presentation: Taiwan
  • Year and Date: 20130000
• [Presentation] Nitrite as a crossroad of multidisciplines : its past, present and future (2013)
  • Author(s): Yamasaki, H.
  • Organizer: The International Symposium on Food, Nutrition and Vascular Function 2013
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 2013-12-03
• [Presentation] NO emission from the water fern Azolla pinata in rapid root abscission (2012)
  • Author(s): Gurung, S., Watanabe, N. S., Cohen, M. F. and Yamasaki, H.
  • Organizer: 第12回日本NO学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20120600
• [Presentation] Characterization of the tropical ornamental tree Codiaeum carigatum cultivars by chlorophyll fluorescence imaging (2011)
  • Author(s): Mollick, A. S. and Yamasaki, H.
  • Organizer: Photosynthetic Research for Sustainability
  • Place of Presentation: Baku, Azerbaijan
  • Year and Date: 20110724-30
• [Presentation] Application of Ficus Microcarpa cv. Golden Leaves to photosynthetic education (2011)
  • Author(s): Heshiki, R. and Yamasaki, H.
  • Organizer: Photosynthetic Research for Sustainability
  • Place of Presentation: Baku, Azerbaijan
  • Year and Date: 20110724-30
• [Remarks]
  • URL: http://www.biology.sci.u-ryukyu.ac.jp