2013 Fiscal Year Final Research Report
Evaluation of the radiation effect on selective chromosome segregation in stem cells
| Project/Area Number |
23310039
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KODAMA Seiji 大阪府立大学, 理学(系)研究科(研究院), 教授 (00195744)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIRAISHI Kazunori 大阪府立大学, 大学院・理学系研究科, 助教 (40347513)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 生物影響 / 幹細胞 |
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| Research Abstract |
We studied an effect of radiation on selective chromosome segregation and the relationship between the number of cells showing selective chromosome segregation and p53 expression in mouse neurosphere cells. The result revealed that the number of cells showing selective chromosome segregation was reduced depending on irradiated X-ray doses. This reduction may be related to the dose-dependent decrease in number of CD133 positive cells. In addition, the cells showing selective chromosome segregation were not observed in p53 knock out (KO) mouse neurosphere cells and the number of those cells was significantly reduced in p53 knock down (KD) neurosphere cells, suggesting that the p53 function is involved in the regulation of selective chromosome segregation.
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[Journal Article] Epigenetic gene silencing is a novel mechanism involved in delayed manifestation of radiation-induced genomic instability in mammalian cells2011
Author(s)
Suzuki, K., Yamaji, H., Kobashigawa, S., Kawauchi, R., Shima, K., Kodama, S., and Watanabe, M.
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Journal Title
Radiat. Res
Volume: 175
Pages: 416-423
DOI
Peer Reviewed
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