High functionality of novel carbonyl sulfide hydrolase aiming at the effective removal of carbonyl sulfide

2013 Fiscal Year Final Research Report

• Project/Area Number: 23310051
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: KATAYAMA Yoko 東京農工大学, (連合)農学研究科(研究院), 教授 (90165415)
• Co-Investigator(Kenkyū-buntansha): ODAKA Masahumi 東京農工大学, 大学院・工学研究院, 准教授 (20224248) ; NOGUCHI Keiichi 東京農工大学, 学内共同利用施設等, 准教授 (00251588)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 硫化カルボニル / 致死性有毒ガス / オゾン層破壊 / 酵素分解処理 / 分子育種 / 結晶構造解析 / 微生物分解

Research Abstract

Carbonyl sulfide hydrolase (COSase) has been isolated from sulfur oxidizing bacterium. COSase catalyses degradation of carbonyl sulfide (COS) that is a trace constituent in the atmosphere and is considered as one of important gas affecting on the global climate especially on ozone depletion. The aim of our research is to obtain information to make highly efficient enzyme molecule so that powerful removal of COS will be possible. We introduced mutations of several amino acids around the sequence of the active center of the enzyme and alpha 5 helix that is a putative tunnel of substrate or reaction product by forming point mutation and examined the effects against COS degrading activity. Furthermore, we tried to isolate new COS degrading microorganisms from soil samples because soil environment is the major sink of atmospheric COS. Novel COS degrading microorganisms including bacteria and fungi were isolated from the soil.

Research Products

• [Journal Article] Carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115 is one of the β-carbonic anhydrase family enzymes (2013)
  • Author(s): Ogawa T, Noguchi K, Saito M, Nagahata Y, Kato H, Ohtaki A, Nakayama H, Dohmae N, Matsushita Y, Odaka M, Yohda M, Nyunoya H, Katayama Y
  • Journal Title: J Am Chem Soc Volume: 135 Pages: 3818-3825
  • DOI: 10.1021/ja307735e
  • Peer Reviewed
• [Journal Article] Addressing the microbiological problems of cultural property and archive documents after earthquake and tsunami (2013)
  • Author(s): Gu JD, Kigawa R, Sato Y, Katayama Y
  • Journal Title: Int Biodeter Biodeg Volume: 85 Pages: 345-346
  • DOI: 10.1016/j.ibiod.2013.08.018
  • Peer Reviewed
• [Journal Article] Cloning and expression of a gene encoding novel thermostable thiocyanate- degrading enzyme from a mesophilic alpha- proteobacteria strain THI201 (2013)
  • Author(s): Hussain A, Ogawa T, Saito M, Sekine T, Nameki M, Matsushita Y, Hayashi T, Katayama Y
  • Journal Title: Microbiol Volume: 159 Pages: 2294-2302
  • DOI: 10.1099/mic.0.063339-0
  • Peer Reviewed
• [Journal Article] Bacterial communities in pigmented biofilms formed on the sandstone bas-relief walls of the Bayon temple, Angkor Thom, Cambodia (2013)
  • Author(s): Kusumi A, Li X, Osuga Y, Kawashima A, Gu JD, Nasu M. Katayama Y
  • Journal Title: Microb Environ Volume: 28 Pages: 422-431
  • DOI: 10.1264/jsme2.ME13033
  • Peer Reviewed
• [Journal Article] Occurrence of Aspergillus allahabadii on sandstone at Bayon Temple, Angkor Thom, Cambodia (2013)
  • Author(s): Hu H, Ding S, Katayama Y. (他5名)
  • Journal Title: Int Biodeter Biodeg Volume: 76 Pages: 112-117
  • DOI: 10.1016/j.ibiod.2012.06.022
  • Peer Reviewed
• [Journal Article] Phylogenetic assessment of culture collection strains of Thiobacillus thioparus, and definitive 16S rRNA gene sequences for T. thioparus, T. denitrificans, and Halothiobacillus neapolitanus (2013)
  • Author(s): Boden, R, Cleland D, Green P N, Katayama Y (他3名)
  • Journal Title: Arch Microbiol Volume: 194 Pages: 187- 195
  • DOI: 10.1007/s00203-011-0747-0
  • Peer Reviewed
• [Journal Article] Vertical distribution of carbonyl sulfide at Mt. Fuji, Japan (2012)
  • Author(s): Kato H, Igarashi Y, Dokiya Y, Katayama Y
  • Journal Title: Water Air Soil Pollut Volume: 223 Pages: 159-167
  • DOI: 10.1007/s11270-011-0847-0
  • Peer Reviewed
• [Journal Article] Mycobacteria isolated from Angkor monument sandstones grow chemolithoautotrophically by oxidizing elemental sulfur (2011)
  • Author(s): Kusumi A, Li X, Katayama Y
  • Journal Title: Front Microbiol Volume: 2 Pages: 104
  • DOI: 10.3389/fmicb.2011.00104
  • Peer Reviewed
• [Presentation] 遺跡劣化砂岩から分離されたFusarium solaniに内生する細菌 (2014)
  • Author(s): 片山葉子
  • Organizer: 第1回菌類内生細菌情報交換会
  • Place of Presentation: 茨城大学(茨城県)
  • Year and Date: 2014-03-21
  • Invited
• [Presentation] 土壌より分離した細菌による硫化カルボニル分解 (2013)
  • Author(s): 猪俣祥
  • Organizer: 第29回日本微生物生態学会大会
  • Place of Presentation: 鹿児島大学(鹿児島県)
  • Year and Date: 2013-11-24
• [Presentation] 大気微量硫黄成分である硫化カルボニルの Trichoderma spp.による分解 (2013)
  • Author(s): 小澤理恵
  • Organizer: 第29回日本微生物生態学会大会
  • Place of Presentation: 鹿児島大学(鹿児島県)
  • Year and Date: 2013-11-24
• [Presentation] アンコール遺跡劣化石材表面のバイオフィルムに蓄積した硝酸とその起源の推定 (2013)
  • Author(s): 片山葉子
  • Organizer: 第29回日本微生物生態学会大会
  • Place of Presentation: 鹿児島大学(鹿児島県)
  • Year and Date: 2013-11-24
• [Presentation] Degradation of hedoro, organically enriched sediment, discharged by tsunami of the great east Japan earthquake in 2011 (2013)
  • Author(s): H IHARA
  • Organizer: The 22th Korea-Japan Symposium on Water Environment 2013
  • Place of Presentation: グランドホテル テグ(大韓民国)
  • Year and Date: 2013-10-21
• [Presentation] 森林および火山ガス発生地域の土壌から分離された真菌の硫化カルボニル分解活性の比較 (2013)
  • Author(s): 小澤理恵
  • Organizer: 日本土壌微生物学会2013年度大会
  • Place of Presentation: 東京農工大学(東京都)
  • Year and Date: 2013-06-20
• [Presentation] 遺跡劣化砂岩から分離されたFusarium solani THIF01f 株の無機塩硫黄培地での生育 (2013)
  • Author(s): 北原直樹
  • Organizer: 日本土壌微生物学会 2013年度大会
  • Place of Presentation: 東京農工大学(東京都)
  • Year and Date: 2013-06-20
• [Presentation] 津波堆積物の性状と微生物叢の好気および嫌気環境下での変化 (2013)
  • Author(s): 猪原英之
  • Organizer: 第48回日本水環境学会年会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2013-03-19
• [Presentation] Microbial deterioration of stones at Angkor site, Cambodia (2012)
  • Author(s): Y. Katayama
  • Organizer: International Symposium on the Conservation and Restoration of Cultural Property 2012
  • Place of Presentation: 上野(東京都)
  • Year and Date: 2012-12-05
  • Invited
• [Presentation] Oxidation of elemental sulfur and chemolithotrophic growth of Fusarium solani strain THIF01 isolated from deteriorated stone of Angkor monuments (2012)
  • Author(s): N. Kitahara
  • Organizer: International Symposium on the Conservation and Restoration of Cultural Property 2012
  • Place of Presentation: 上野(東京都)
  • Year and Date: 2012-12-05
• [Presentation] Degradation of atmospheric COS by Trichoderma sp. strain KSF8 (2012)
  • Author(s): R Ozawa
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 豊橋技術科学大学(愛知県)
  • Year and Date: 2012-09-10
• [Presentation] Microbial growth and oxidation of elemental sulfur on deteriorated sandstones in Angkor monuments, Cambodia (2012)
  • Author(s): Y. Katayama
  • Organizer: Chemical and ecological researches on the microorganisms for preserving cultural properties in both Japan and Hungary
  • Place of Presentation: 奈良市(奈良県)
  • Year and Date: 2012-07-07
  • Invited
• [Presentation] Carbonyl sulfide hydrolase : a novel β-carbonic anhydrase family enzyme catalyzing ambient level of carbonyl sulfide (2012)
  • Author(s): T Ogawa
  • Organizer: 12th Japan-China-Korea Joint Symposium on Enzyme Engineering(日中韓酵素工学会議)
  • Place of Presentation: (日中韓酵素工学会議)
  • Year and Date: 2012-05-28
• [Book] よみがえる富士山測候所 2012年 (2012)
  • Author(s): 大河内博,緒方裕子,皆巳幸也,片山葉子,米持真一
  • Total Pages: 94-100
  • Publisher: 成山堂書店
• [Remarks] 国立大学法人東京農工大学科学技術展2013土壌に生息する微生物による空気中の硫化カルボニルの分解 猪俣祥,小澤理恵,片山葉子
• [Remarks] 国立大学法人東京農工大学科学技術展2012 土壌微生物のはたらき‐空気中の微量硫黄ガスの分解‐小澤理恵,猪俣祥,加藤広海,片山葉子
• [Remarks] 国立大学法人東京農工大学科学技術展2011 効率的除去を目指した新奇硫化カルボニル分解酵素の高機能化 片山葉子,野口恵一,小川貴弘,尾高雅文