2013 Fiscal Year Final Research Report
Measurement of local viscosity of the hydration shell of protein by time-resolved fluorescence anisotropy spectroscopy
| Project/Area Number |
23310071
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Tohoku University |
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Principal Investigator |
SUZUKI MAKOTO 東北大学, 工学(系)研究科(研究院), 教授 (60282109)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Nobuyuki 東北大学, 大学院工学研究科, 准教授 (00313263)
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| Co-Investigator(Renkei-kenkyūsha) |
WAZAWA Tetsuichi 東北大学, 大学院工学研究科, 研究支援者 (80359851)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 局所粘度計測 / 蛍光偏光解消 / 回転相関時間 / ハイパーモバイル水 / アクチンフィラメント / 蛍光異方性 / 水和水 / 水和水の粘度 |
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| Research Abstract |
In this study, rotational correlation time Tc of fluorescent dye was measured to evaluate the local viscosity of the hydration region of protein surface.The Tc measured by this method was found to be in linear relation with the solvent viscosity. The CW-laser-based method revealed shorter Tc of Cy3 linked at Cys374 of actin in F-actin than around G-actin, which is consistent with the result by dielectric relaxation data.On the other hand,Tc (<1ns) of small fluorescent dye such as rhodamin 6G in water could not be measured by pulse-laser-based method without picosecond pulse width.We have designed a frequency-region fluorescent anisotropy spectroscopic method to measure Tc of small fluorescent dyes.
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Research Products
(24 results)
