Structural Changes by Plasma Membrane Differentiation

2013 Fiscal Year Final Research Report

• Project/Area Number: 23310087
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: MORONE Nobuhiro 京都大学, 物質-細胞統合システム拠点, 講師 (50399680)
• Co-Investigator(Kenkyū-buntansha): HEUSER John 京都大学, 物質-細胞統合システム拠点, 教授 (40571815)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 細胞膜 / 細胞 / 幹細胞 / 分化 / カベオラ / アクチン膜骨格 / クラスリン被覆ピット / 電子顕微鏡法

Research Abstract

Cellular morphology of tissue stem cells is dramatically changed during the differentiation process from progenitors toward matures. The interaction between plasma membranes and cytoskeletons, the structural networks of actin-based membrane skeleton, caveolae, and clathrin-coated pits at the interface with the plasma membrane play crucial roles. By rapid-freeze, freeze-replica electron microscopy, we carefully visualized the cytoplasmic surface of those structures, and particularly got the novel findings about caveolar filaments and crescent-like structures. Also the other researches regarding the plasma membrane differentiation are going on.

Research Products

• [Journal Article] Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging (2013)
  • Author(s): Nishimura H, Ritchie K, Kasai RS, Morone N, Sugimura H, Tanaka K, Sase I, Yoshimura A, Nakano Y, Fujiwara TK, and Kusumi A
  • Journal Title: J Cell Biol. Volume: 202 Pages: 967-83
  • DOI: 10.1083/jcb.201301053
• [Journal Article] Development of a reentrant arrhythmia model in human pluripotent stem cell-derived cardiac cell sheets (2012)
  • Author(s): Kadota S, Minami I, Morone N, Heuser JE, Agladze K, Nakatsuji N
  • Journal Title: Eur Heart J. Volume: 34(15) Pages: 1147-56
  • DOI: 10.1093/eurheartj/ehs418
• [Journal Article] A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions (2012)
  • Author(s): Minami I, Yamada K, Otsuji TG, Yamamoto T, Shen Y, Otsuka S, Kadota S, Morone N, Barve M, Asai Y, Tenkova-Heuser T, Heuser JE, Uesugi M, Aiba K, Nakatsuji N
  • Journal Title: Cell Rep Volume: 2(5) Pages: 1448-60
  • DOI: 10.1016/j.celrep.2012.09.015
• [Journal Article] Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery (2012)
  • Author(s): Tanaka T, Takahashi K, Yamane M, Tomida S, Nakamura S, Oshima K, Niwa A, Nishikomori R, Kambe N, Hara H, Mitsuyama M, Morone N, Heuser JE, Yamamoto T, Watanabe A, Sato-Otsubo A, Ogawa S, Asaka I, Heike T, Yamanaka S, Nakahata T, Saito MK
  • Journal Title: Blood Volume: 120(6) Pages: 1299-308
  • DOI: 10.1182/blood-2012-03-417881
• [Journal Article] Membrane mechanisms for signal transduction : the coupling of the meso-scale raft domains to membrane-skeleton-induced compartments and dynamic protein complexes (2012)
  • Author(s): Kusumi A, Fujiwara TK, Morone N, Yoshida KJ< Chadda R, Xie M, Kasai RS, Suzuki KG
  • Journal Title: Semin Cell Dev Biol. Volume: 23(2) Pages: 126-44
  • DOI: 10.1016/j.semcdb.2012.01.018
• [Journal Article] Cells respond to mechanical stress by rapid disassembly of caveolae (2011)
  • Author(s): Sinha B, Koster D, Ruez R, Gonnord P, Bastiani M, Abankwa D, Stan RV, Butler-Browne G, Vedie B, Johannes L, Morone N, Parton RG, Raposo G, Sens P, Lamaze C, Nassoy P
  • Journal Title: Cell. Volume: 144(3) Pages: 402-13
  • DOI: 10.1016/j.cell.2010.12.031
• [Presentation] Three findings by electron microscopy in caveolar research (2013)
  • Author(s): Morone N
  • Organizer: JSM-MPFI Joint-Seminar 2013
  • Place of Presentation: Max Plang Florida Institute for Neuroscience, FL, USA
  • Year and Date: 2013-12-27
• [Presentation] Dendra融合タンパク質によるカベオラの急速凍結・ディープエッチ電子顕微鏡法 (2013)
  • Author(s): 諸根信弘、吉村安寿弥、ホイザー・ジョン
  • Organizer: 日本顕微鏡学会第69回学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2013-05-20
• [Presentation] Cavin regulates caveolar endocytosis by forming crescents that girdle caveolae, stabilizes then in relatively flat conformations, and links them to actin-membrane skeleton (2012)
  • Author(s): Morone N
  • Organizer: 2012 Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-12-17
• [Presentation] Plasma membrane differentiation during adipogenesis : Freeze-etch electron microscopy for caveolar architecture (2012)
  • Author(s): Morone N
  • Organizer: JSM-NUS Joint-Seminar 2011
  • Place of Presentation: National University of Singapore, Singapore
  • Year and Date: 2012-01-13
• [Presentation] カベオラ動態の細胞電子顕微鏡解析 (2011)
  • Author(s): 諸根信弘
  • Organizer: 第63回日本細胞生物学会大会(ワークショップ)
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-06-27
• [Book] 「透過型電子顕微鏡法:組織・細胞の3次元メゾスケール構造解析」(先端バイオマテリアルハンドブック) (2012)
  • Author(s): 和久井文、河野朋子、青山一弘、諸根信弘
  • Total Pages: 96-102
  • Publisher: 株式会社エヌ・ティー・エス
• [Remarks]
  • URL: http://www.icems.kyoko-u.ac.jp/j/ppl/grp/heuser.html