2013 Fiscal Year Final Research Report
Study on Molecular Mechanism of Cytotoxicity of Marine Natural Products
| Project/Area Number |
23310148
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KIGOSHI Hideo 筑波大学, 数理物質系, 教授 (90169839)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Ichiro 筑波大学, 数理物質系, 助教 (20375413)
KITA Masaki 筑波大学, 数理物質系, 准教授 (30335012)
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| Co-Investigator(Renkei-kenkyūsha) |
UESUGI Motonari 京都大学, 物質-細胞統合システム拠点, 教授 (10402926)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Keywords | アプリロニンＡ / アクチン / チューブリン / 海洋天然物 / 三元複合体 / アポトーシス / ビセライドＡ / 全合成 |
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| Research Abstract |
The molecular mechanism of antitumor activity of aplyronine A, a marine macrolide, has been investigated. Using chemical probes aplyronine A was found to bind to actin and tubulin to form a ternary complex.
As a result, aplyronine A inhibits tubulin polymerization, induces irregular, multipolar spindle structures, inhibits cell-cycle progression in M-phase, and induces apoptosis.
Total synthesis of biselide A, a cytotoxic marine macrolide, was achieved by using Wittig reaction and sereoselective enzymatic hydrolysis of allylic alcohol as key steps.
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[Journal Article] Fluorescent Aplyronine A : Intracellular Accumulation and Disassembly of Actin Cytoskeleton in Tumor Cells2012
Author(s)
Masaki Kita, Kozo Yoneda, Yuichiro Hirayama, Kota Yamagishi, Yuki Saito, Yuka Sugiyama, Yoshihiro Miwa, Osamu Ohno, Maho Morita, Kiyotake Suenaga, Hideo Kigoshi
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Journal Title
ChemBioChem
Volume: 13 (12)
Pages: 1754-1758
DOI
Peer Reviewed
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[Journal Article] Aplyronines D-H from the sea hare Aplysia kurodai : isolation, structures, and cytotoxicity2012
Author(s)
Makoto Ojika, Hideo Kigoshi, Kiyotake Suenaga, Yoshifumi Imamura, Kohji Yoshikawa, Takeshi Ishigaki, Akira Sakakura, Tsuyoshi Mutou, and Kiyoyuki Yamada
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Journal Title
Tetrahedron
Volume: 68 (4)
Pages: 982-987
DOI
Peer Reviewed
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[Presentation] Synthetic studies of biselide E2012
Author(s)
Shota Funakubo, Yuto Onozaki, Dai Kawamura,Mami Shimanuki, Takuya Yamada, Ichiro Hayakawa, Hideo Kigoshi
Organizer
3nd Symposium on Creation of Functional Materials supported by Strategic Initiative A, University of Tsukuba
Place of Presentation
筑波大学つくばキャンパス(つくば市)
Year and Date
2012-12-10
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[Presentation] Synthetic studies of biselides A and B2012
Author(s)
Mami Shimanuki, Shota Funakubo, Takuya Yamada, Kizuku Kimura, Yohsuke Satoh, Ichiro Hayakawa, Hideo Kigoshi
Organizer
3nd Symposium on Creation of Functional Materials supported by Strategic Initiative A, University of Tsukuba
Place of Presentation
筑波大学つくばキャンパス(つくば市)
Year and Date
2012-12-10
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