2013 Fiscal Year Final Research Report
How is the genome integrity of the hyperthermophiles protected and propagated?
Project/Area Number |
23310152
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Living organism molecular science
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Research Institution | Kyushu University |
Principal Investigator |
ISHINO YOSHIZUMI 九州大学, (連合)農学研究科(研究院), 教授 (30346837)
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Co-Investigator(Kenkyū-buntansha) |
ATOMI Haruyuki 京都大学, 大学院・工学研究科, 教授 (90243047)
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Project Period (FY) |
2011-04-01 – 2014-03-31
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Keywords | アーキア / DNA複製 / 極限環境生命 |
Research Abstract |
Proteins and enzymes involved in DNA replication and repair in the hyperthermophilic archaea, Thermococcus kodakarensis and Pyrococcus furious were characterized by both biochemical and genetical techniques. There are three Mcm and two PCNA in T. kodakarensis, and to understand the functional sharing, we characterized the purified Mcm and PCNA proteins. In addition, we isolated amutant T. kodakarensis strains, in which one of these genes wa knocked out, and characterized their phenotypes. We found that Mcm3 and PCNA1 are essential for the viability of T. kodakarensis. Exact functions of Mcm1, Mcm2, and PCNA2 remain to be elucidated. We searched for enzymes involved in the damaged base repair system in the hyperthermophilic archaea. We identified a novel endonuclease, which cleaves at 5'-side of the damaged nucleotide, hypoxanthine, uracil, xanthine, and AP site in the DNA strand. We designated it Endonuclease Q and applied for a patent as a novel substance from Kyushu University.
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[Journal Article] A novel single-strand specific 3'-5' exonuclease found in the hyperthermophilic archaeon,, Pyrococcus furiosus2013
Author(s)
Tori, K., Ishino, S., Kiyonari, S., Tahara, S. and Ishino, Y
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Journal Title
PLoS One
Volume: 8
Pages: e58497
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[Journal Article] Comparative analyses of the two PCNAs from the hyperthermophilic archaeon, Thermococcus kodakarensis2012
Author(s)
Kuba, Y., Ishino, S., Yamagami, T., Tokuhara, M., Kanai, T., Fujikane, R.,Daiyasu., Atomi, H., Ishino, Y.
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Journal Title
Genes Cells
Volume: 17
Pages: 923-937
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[Journal Article] Biochemical and genetical analyses of the three mcm genes from the hyperthermophilic archaeon, Thermococcus kodakarensis2011
Author(s)
Ishino, S., Fujino, S., Tomita, H., Ogino, H., Takao, K., Daiyasu, H., Kanai, T., Atomi, H., and Ishino, Y.
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Journal Title
Genes Cells
Volume: 16
Pages: 1176-1189
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[Journal Article] The GINS complex from the thermoacidophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication2011
Author(s)
Ogino, H., Ishino, S. Mayanagi, K., Haugland,G, H., Birkeland, N-K., Yamagishi, A., and Ishino Y
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Journal Title
Extremophiles
Volume: 15
Pages: 529-539
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[Journal Article] Architectures of archaeal GINS complexes, essential DNA replication initiation factors2011
Author(s)
Oyama, T, Ishino, S, Fujino, S, Ogino, H, Shirai, T, Mayanagi, K, Saito, M, Nagasawa, N, Ishino, and Y, Morikawa, K.
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Journal Title
BMC Biol
Volume: 9
Pages: 28
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[Journal Article] Reverse-Chaperoning Activity of an AAA+ Protein2011
Author(s)
Liu, C., McKinney, MC., Chen, YH., Earnest, TM., Shi, X., Lin, LJ., Ishino, Y., Dahmen, K., Cann, I.K., Ha, T
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Journal Title
Biophys J
Volume: 100
Pages: 1344-1352
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[Journal Article] Architecture of the DNA polymerase B-proliferating cell nuclear antigen (PCNA)-DNA ternary complex2011
Author(s)
Mayanagi, K., Kiyonari, S., Nishida, H., Saito, M., Kohda, D., Ishino, Y., Shirai, T., and Morikawa, K
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 108
Pages: 1845-1849
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[Presentation] The synergistic stimulation of the MCM activity by Cdc6/Orc1 and GINS homotetramer from the archaeon, Thermoplasma acidophilum2012
Author(s)
Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Takuji Oyama, Tsuyoshi Shirai, Kosuke Morikawa, Gyri Teien Haugland, Nils-Kåre Birkeland, and Yoshizumi Ishino
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡国際会議場,福岡
Year and Date
20121211-14
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[Presentation] Divergently evolved replicative helicase from the archaeon2012
Author(s)
Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Takuji Oyama, Tsuyoshi Shirai, Kosuke Morikawa, Gyri Teien Haugland, Nils-Kåre Birkeland, and Yoshizumi Ishino
Organizer
Thermoplasma acidophilum The 8th 3R symposium
Place of Presentation
Awaji Yumebutai International Conference Center, Japan
Year and Date
20121125-28
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