2013 Fiscal Year Final Research Report
Development of high throughput screening method of G-quadruplexes using small molecules
| Project/Area Number |
23310158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
NAGASAWA Kazuo 東京農工大学, 工学(系)研究科(研究院), 教授 (10247223)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | グアニン四重鎖 / テロメア / DNAマイクロアレイ / テロメスタチン / 蛍光プローブ |
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| Research Abstract |
G-Quadruplexes (G4s) are three-dimensional structures that are formed in guanine-rich polynucleotides. Relatively few G4-forming oligonucleotides (GFOs) have been identified so far, but they are important because they play significant roles in regulation of gene expression and replication. Computational analysis suggests huge numbers of GFOs are present in the genome. However, there has been no simple and direct screening methodology for large-scale screening of GFOs.
In this project, we have developed a high-throughput screening method for GFOs by using the fluorescent G4 ligand L1Cy5-7OTD with a DNA microarray. With this method, we found 1,998 novel GFOs in a CpG island (CGI) microarray bearing 88,737 targets. Gene Ontology (GO) analysis suggests that these GFOs were mainly associate with metabolic processes, transcriptional regulation, biosynthetic processes and developmental processes. The present method would be easily applicable to other DNA microarrays.
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Research Products
(37 results)
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[Presentation] Devepolment of the Caged G-Quadruplex Ligand2011
Author(s)
Takahiro Nakamura, Keisuke Iida, Masayuki Tera, Hiroyuki Seimiya, Kazuo Shin-ya, Kazuo Nagasawa
Organizer
Third International Meeting on G-Quadruplex and G-assembly
Place of Presentation
Grand Hotel Vesuvio (Naples, Italy)
Year and Date
20110628-1202
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