2013 Fiscal Year Final Research Report
Modular RNA engineering of RNA molecular switches (riboswitches)
Project/Area Number |
23310161
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Chemical biology
|
Research Institution | University of Toyama (2013) Kyushu University (2011-2012) |
Principal Investigator |
IKAWA YOSHIYA 富山大学, 理工学研究部(理学), 教授 (70281087)
|
Co-Investigator(Renkei-kenkyūsha) |
FURUTA Hiroyuki 九州大学, 大学院・工学研究院, 教授 (40244157)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Keywords | リボスイッチ / RNA / モジュール工学 / ケミカルバイオロジー / リボザイム / シンセティック・バイオロジー / 合成生物学 |
Research Abstract |
Riboswitches are attractive not only as drug targets for novel antibiotics but also as modular tools for controlling gene expression. Riboswitches frequently have GNRA loop/receptor interaction modules in thier aptamer modules. In this study, the functional importance of the GAAA loop-receptor interaction in the c-diGMP riboswitch was examined. A series of variant c-diGMP riboswitches with mutations in the GAAA loop/receptor interaction were assayed for their switching abilities. These analyses strongly suggested that the GAAA loop/receptor interaction does not simply establish the RNA 3D structure but docking of P2 GAAA loop rigidifies the P3 element. This mechanism was applied to design an artificial riboswitch bearing a theophylline aptamer module in P3 the structural rigidity of which could be modulated by the small molecule theophylline. We also generated a new class of aptamer module that specifically recognizes a GAAC RNA tetraloop by employing in vitro directed evolution.
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Research Products
(25 results)