2013 Fiscal Year Final Research Report
Studies on inhibition mechanism of synchronized bursts in neuronal network
| Project/Area Number |
23350001
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
UCHIDA Tsutomu 北海道大学, 工学(系)研究科(研究院), 准教授 (70356575)
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| Co-Investigator(Kenkyū-buntansha) |
GOHARA Kazutoshi 北海道大学, 大学院・工学研究院, 教授 (40153746)
NAGAYAMA Masafumi 北海道教育大学, 教育学部, 准教授 (70374585)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 生物物理化学 / 麻酔 / ニューラルネットワーク / 電気信号測定 / 分光測定 |
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| Research Abstract |
In order to understand the mechanism of general anesthesia with Xe, we carried out the mesoscopic and microscopic measurements on cultured neuronal network from rat cortex. We measured the activity depression of cultured neuronal network under Xe gas pressure by multi-electrode arrays (mesoscopic measurement) and by a Raman spectroscopy (microscopic measurement). When we applied Xe gas between 0.1 and 0.5 MPa on cultured neuronal network, the characteristic firing activity (synchronized burst firing) was completely inhibited under the conditions of Xe pressure higher than 0.3 MPa. Raman spectra of neurons showed the peak shift of C-H stretching mode of the cell membranes under Xe pressure, which indicated that Xe molecules dissolved in membranes. This indication coincides with that suggested by MD simulation.
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[Journal Article] Cell Patterning Using a Template of Micro-structured Organosilane Layer Fabricated by Vacuum Ultraviolet Light Lithography2011
Author(s)
M. Yamaguchi, K. Ikeda, M. Suzuki, A. Kiyohara, S. N. Kudoh, K. Shimizu, T. Taira, D. Ito, T. Uchida, K. Gohara
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Journal Title
Langmuir
Volume: Vol. 27, No. 20
Pages: 12521-12532
DOI
Peer Reviewed
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