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2013 Fiscal Year Final Research Report

Research on Full-protocol Disease Marker Screening System using Integrated Lab-on-a-CD for Immunoassay

Research Project

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Project/Area Number 23350035
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionUniversity of Hyogo

Principal Investigator

UTSUMI Yuichi  兵庫県立大学, 高度産業科学技術研究所, 教授 (80326298)

Co-Investigator(Kenkyū-buntansha) TAKEO Masahiro  兵庫県立大学, 大学院・工学研究科, 准教授 (40236443)
YUSA Shinichi  兵庫県立大学, 大学院・工学研究科, 准教授 (00301432)
UKITA Yoshiaki  北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (40578100)
KATAOKA Masatoshi)  産業技術総合研究所, 健康工学研究部門, グループ長 (20224438)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywordsチップ分析 / 生体分析 / 免疫反応 / バイオリアクター / ELISA / マイクロ化学システム / Lab-on-a-chip
Research Abstract

This paper reports a new type of lab-on-a-CD device with three-dimensional microchannel networks and vertical capillary bundle structure. The device consist of the multiple lab-on-a-CD chips with conventional planer microchannels and vertical microchannels which constructed on the disks made of poly-dimethylsiloxane (PDMS) and poly-methylmethacrilrate (PMMA). The PMMA layer with vertical channel were fabricated by deep X-ray lithography using synchrotron radiation. We designed and fabricated the chips for aim of taking enzyme-linked immunosorbent assay(ELISA). Three-dimensional liquid transportation through Lab-on-a-CD structure is suggested by computational fluid dynamics and it is successfully demonstrated chips. Detection of immunogroblin G(IgG) from mouse is successfully demonstrated in the three-dimensional devices and it is notable that the result suggests quite high-sensitive implying detection limit down to 1ng/mL by primitive result.

  • Research Products

    (14 results)

All 2014 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (9 results)

  • [Journal Article] Optofluidic Devices with Surface-Enhanced Raman Scattering Active Three- Dimensional Gold Nanostructure2013

    • Author(s)
      Ryo Takahashi, Takao Fukuoka, Yuichi Utsumi, Akinobu Yamaguchi
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 52 Pages: 06GK12,1-5

    • Peer Reviewed
  • [Journal Article] Application of photo-etching of polytetrafluoroethylene induced by high energy synchrotron radiation to LIGA2013

    • Author(s)
      Hideki Kido, Tomoyuki Kuroki, Masaaki Okubo, Yuichi Utsumi
    • Journal Title

      Microsystem Technologies

      Volume: 19, 3 Pages: 301-307

    • Peer Reviewed
  • [Journal Article] Stacked centrifugal microfluidic device with three-dimensional microchannel networks and multifunctional capillary bundle structures for immunoassay2012

    • Author(s)
      Yoshiaki Ukita, Saki Kondo, Tsukasa Azeta, Masaki Ishizawa, Chiwa Kataoka, Masahiro Takeo, Yuichi Utsumi
    • Journal Title

      Sensors and Actuators B : Chemical

      Volume: 166-167 Pages: 898-906

    • Peer Reviewed
  • [Journal Article] Proposal of High-Integrated Three-Dimensional Microfluidic by Using Centrifugal Force for Enzyme Linked Immunosorbent Assay2012

    • Author(s)
      Tsukasa Azeta, Yuichi Utsumi
    • Journal Title

      Journal of Japan Institute of Electronics packaging

      Volume: 15, 1 Pages: 38-41

    • Peer Reviewed
  • [Journal Article] A Novel Micromixer with Three-Dimensionally Cross-Linked Capillary Array Structure Fabricated by Deep X-ray Lithography2011

    • Author(s)
      Saki Kondo, Yoshiaki Ukita, Kuniyo Fujiwara, Yuichi Utsumi
    • Journal Title

      Electrical Engineering in Japan

      Volume: 177, 1 Pages: 26-31

    • Peer Reviewed
  • [Presentation] Novel Pressure-Controlling Valve of Centrifugal Microfluidics2014

    • Author(s)
      Masaki Ishizawa, Yasuto Arisue, Ikuo Okada, Akinobu Yamaguchi, Yuichi Utsumi
    • Organizer
      PITTCON2014
    • Place of Presentation
      Chicago, USA
    • Year and Date
      20140302-06
  • [Presentation] Blood separation lab-on-a-CD with controlled surface2013

    • Author(s)
      Hiroki Nose, Masaki Ishizawa, Takeshi Kunisada, Kazuhisa Kuroda, Akinobu Yamaguchi, Yuichi Utsumi
    • Organizer
      7th International Conference on Molecular Electronics and Bioelectronics (M&BE7)
    • Place of Presentation
      福岡国際会議場, Fukuoka, Japan
    • Year and Date
      20130317-19
  • [Presentation] Contamination-Free Internally-Triggered Automatic Flow Sequencing for Microfluidics2012

    • Author(s)
      Masaki Ishizawa, Yuichi Utsumi
    • Organizer
      38th International Conference on Micro and Nano Engineering (MNE2012)
    • Place of Presentation
      Toulouse, France
    • Year and Date
      20120916-20
  • [Presentation] Visualization of Biological Fluid Behaviour on Spinning Centrifugal Microfluidics using Coaxial Micro-Stroboscope2012

    • Author(s)
      Yoshiaki Ukita, Takayuki Oguro, Masaki Ishizawa, Hiroki Nose, Yuichi Utsumi, Yuzuru Takamura
    • Organizer
      38th International Conference on Micro and Nano Engineering (MNE2012)
    • Place of Presentation
      Toulouse, France
    • Year and Date
      20120916-20
  • [Presentation] Three-Dimensional Lab-on-a-CD with Enzyme-Linked Immunosorbent Assay2012

    • Author(s)
      Masaki Ishizawa, Tsukasa Azeta, Hiroki Nose, Yoshiaki Ukita, Yuichi Utsumi
    • Organizer
      Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2012)
    • Place of Presentation
      京都大学, Kyoto, Japan
    • Year and Date
      20120305-08
  • [Presentation] Multi Stacked Centrifugal Microfluidics for Enzyme-linked Immunosorbent Assay2011

    • Author(s)
      Masaki Ishizawa, Tsukasa Azeta, Hiroki Nose, Yoshiaki Ukita, Masahiro Takeo, Kyuya Nakagawa, Shinichi Yusa, Yuichi Utsumi
    • Organizer
      37th International Confedence on Micro and Nano Engineering (MNE2011)
    • Place of Presentation
      Berlin, Germany
    • Year and Date
      20110919-23
  • [Presentation] Proposal of Three-dimensional Microfluidics Device For Immunoassay Using Centrifugal Force2011

    • Author(s)
      Tsukasa Azeta, Yoshiaki Ukita, Yuichi Utsumi
    • Organizer
      9th International Workshop on High Aspect Ratio Micro Structure Technology (HARMST2011)
    • Place of Presentation
      Hsin Chu, Taiwan
    • Year and Date
      20110612-18
  • [Presentation] Three-Dimensional Lab-on-a-CD with Enzyme-Linked Immunosorbent Assay2011

    • Author(s)
      Tsukasa Azeta, Yoshiaki Ukita, Masahiro Takeo, Kyuya. Nakagawa, Shinichi Yusa, Yuichi Utsumi
    • Organizer
      9th International Workshop on High Aspect Ratio Micro Structure Technology (HARMST2011)
    • Place of Presentation
      Hsin Chu, Taiwan
    • Year and Date
      20110612-18
  • [Presentation] Environmental Analysis using Stacked Centrifugal Microfluidicsy2011

    • Author(s)
      Yoshiaki Ukita, Tsukasa Azeta, Saki Kondo, Chiwa Kataoka, Shinichi Yusa, Masahiro Takeo, Yuichi Utsumi
    • Organizer
      9th International Workshop on High Aspect Ratio Micro Structure Technology (HARMST2011)
    • Place of Presentation
      Hsin Chu, Taiwan
    • Year and Date
      20110612-18

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Published: 2015-06-25  

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