2013 Fiscal Year Final Research Report
Multifunctional two-photon excitable fluorescence probes for monitoring mitochondrial dynamics
| Project/Area Number |
23350068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional materials chemistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KAWAMATA JUN 山口大学, 医学(系)研究科(研究院), 教授 (40214689)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Yasutaka 山口大学, 大学院・医学系研究科, 助教 (30634753)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 非線形光学 / 二光子吸収 / 二光子励起蛍光顕微鏡 / 蛍光プローブ / 蛍光イメージング / 蛍光 |
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| Research Abstract |
Several fluorescence probes for imaging of live mitochondria have been synthesized. These probes exhibited not only salient two-photon excitation fluorescence efficiencies but also high solubility to water. Owing to the high solubility to water of the novel probes, living cells have been stained without using any organic solvent. This advantage of the present probes enabled long time live cell imaging over than one week with a prominent sensitivity.
In addition, we have developed fluorescence probes which switch accumulated organelle by external stimuli. The probes accumulated at mitochondria when the mitochondrial membrane potential was high. As the cell activity decreased the probes moved from mitochondria to nucleus. Reversible change of accumulated organelle has also been confirmed.
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[Journal Article] Incorporation of cationic electron donor of Ni-pridyltetrathiafulvalene with anionic electron accepter of polyoxometalate2012
Author(s)
R. Tsunashima, T. Matsumoto, N. Hoshino, W. Niiho, M. Kimura, K. Kendo, Y. Suyama, Y. Nishioka, J. Kawamata, S. Noro, T. Nakamura, T, Akutagawa, K. Ishiguro
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Journal Title
Dalton Trans
Volume: 41
Pages: 10060
Peer Reviewed
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