2013 Fiscal Year Final Research Report
Structure and function of sensor proteins that regulate biological functions by gas molecules
| Project/Area Number |
23350084
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Okazaki Research Facilities, National Institutes of Natural Sciences |
|---|
Principal Investigator |
AONO Shigetoshi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (60183729)
|
|---|
| Project Period (FY) |
2011-11-18 – 2014-03-31
|
| Keywords | センサータンパク質 / ヘムタンパク質 / 転写調節因子 / 走化性制御系 / シグナルトランスデューサー |
|---|
| Research Abstract |
A chemotaxis signal transducer protein Aer2 from Pseudomonas aeruginosa was found to be a novel heme-based oxygen sensor protein. The crystal structure of Aer2 was determined, which reveals that a hydrogen bonding interaction between heme-bound oxygen and Trp283 in the distal heme pocket plays an important role for oxygen sensing and signal transduction of Aer2.
A novel heme sensor protein HrtR was also found in this work. The molecular mechanisms of heme sensing by HrtR and of the heme-responsive functional regulation of HrtR were elucidated by determining its crystal structure.
|
