Development and application of Giga-screening system of by-molecules using cell-free biochemical reactions

2014 Fiscal Year Final Research Report

• Project/Area Number: 23360367
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Nagoya University
• Principal Investigator: NAKANO Hideo 名古屋大学, 生命農学研究科, 教授 (00237348)
• Co-Investigator(Kenkyū-buntansha): IWASAKI Yugo 名古屋大学, 大学院生命農学研究科, 准教授 (50273214) ; KOJIMA Takaaki 名古屋大学, 大学院生命農学研究科, 助教 (40909080) ; KOBAYASHI Isao 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所, 研究員 (70425552)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 無細胞タンパク質合成系 / エマルジョン / タンパク質 / 酸化還元酵素 / RNAポリメラーゼ / 一分子PCR / マイクロビーズ

Outline of Final Research Achievements

Creation of bio-macromolecules such as proteins, DNA and RNA is important technology both for bio-industries and bioscience, however, functional design of these molecules are generally impossible due to these complexities. In this research, we have developed a high-throughput library construction and screening system enabling giga-scale screening of such molecules using pL volume droplets in emulsion as reaction chamber for these molecules based on biochemical functions such as manganese peroxidase, horseradish peroxidase, and the substrate peptides and promoter sequences of transglutaminase and RNA polymerase, respectively.
In addition, hand-made device for making uniform droplets in emulsion formation in a short-time has been also developed to improve the screening efficiency.

Free Research Field

生物工学