2013 Fiscal Year Final Research Report
Next generation protein production on cellular quality-control process
| Project/Area Number |
23360371
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
OMASA Takeshi 徳島大学, ソシオテクノサイエンス研究部, 教授 (00252586)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ONITSUKA Masayoshi 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (80571174)
SHIRAI Akihiro 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (40380117)
MASEDA Hideaki 徳島大学, 大学院・ソシオテクノサイエンス研究部, 准教授 (10372343)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | 小胞体ストレス応答 / チャイニーズハムスター卵巣細胞 / 蛋白質医薬品 / 糖鎖 / 細胞培養 |
|---|
| Research Abstract |
Therapeutic antibody is workhorse for the development of pharmaceutical industries. In Japan, more than quarter of biopharmaceuticals was produced using CHO cell line. Recently, over 10 g/L antibody production was reported using CHO cell line. In this project, we focused on the cellular quality-control system for protein secretion process in CHO cell. On the basis of quality-control mechanism, we constructed the improved protein production system.
|
-
-
-
-
-
-
[Journal Article] Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α 2,6-sialytransferase derived from Chinese hamster ovary cells2012
Author(s)
Masayoshi Onitsuka, Wook-Dong Kim, Hiroyuki Ozaki, Akira Kawaguchi, Kohsuke Honda, Hiroyuki Kajiura, Kazuhito Fujiyama, Ryutato Asano, Izumi Kumagai, Hisao Ohtake, Takeshi Omasa
-
Journal Title
Applied Microbiology and Biotechnology
Volume: Vol. 94
Pages: 69-80
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
