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2013 Fiscal Year Final Research Report

The molecular mechanisms of heterochromatin establishment and maintenance

Research Project

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Project/Area Number 23370004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionNagoya City University (2012-2013)
The Institute of Physical and Chemical Research (2011)

Principal Investigator

NAKAYAMA Jun-ichi  名古屋市立大学, システム自然科学研究科, 准教授 (60373338)

Co-Investigator(Renkei-kenkyūsha) HAYASHI Aki  独立行政法人理化学研究所, クロマチン動態研究チーム, 研究員 (80399534)
Project Period (FY) 2011-04-01 – 2014-03-31
Keywords遺伝子 / 発現制御 / ヘテロクロマチン / RNA干渉 / 分裂酵母
Research Abstract

In this research program, we tried to gain a better understanding of the molecular mechanisms underlying the establishment and maintenance of higher-order chromatin structure, heterochromatin. Using fission yeast as a model organism, we employed a genetic screening and successfully identified two novel RNAi factors, Ers1 and Dsh1, involved in heterochromatin assembly. Further analyses uncovered that these factors play roles in recruiting RNAi-effector complexes, such as RDRC, and promote siRNA production to form higher-order chromatin structure.

  • Research Products

    (30 results)

All 2014 2013 2012 2011 Other

All Journal Article (11 results) Presentation (11 results) Book (7 results) Remarks (1 results)

  • [Journal Article] Biochemical and structural properties of Heterochromatin Protein 1 : understanding its role in chromatin assembly2014

    • Author(s)
      Nishibuchi G & Nakayama J
    • Journal Title

      J. Biochem

      Volume: (in press)

    • DOI

      10.1093/jb/mvu032

  • [Journal Article] Spt6 prevents transcription- coupled loss of posttranslationally modified histone H32013

    • Author(s)
      Kato H, Okazaki K, Iida T, Nakayama J, Murakami Y, & Urano, T
    • Journal Title

      Sci Rep

      Volume: 3 Pages: 2186

    • DOI

      10.1038/srep02186

  • [Journal Article] Single cell visualization of yeast gene expression shows correlation of epigenetic switching between multiple heterochromatic regions through multiple generations2013

    • Author(s)
      Mano Y, Kobayashi T, Nakayama J, Uchida H, & Oki M
    • Journal Title

      PLoS Biol

      Volume: 11 Pages: e1001601

    • DOI

      10.1371/journal.pbio.1001601

  • [Journal Article] C-terminus of the Sgf73 subunit of SAGA and SLIK is important for retention in the larger complex and for heterochromatin boundary function2013

    • Author(s)
      Kamata K, Hatanaka A, Goswami G, Shinmyozu K, Nakayama J, Urano T, Hatashita M, Uchida H, & Oki M
    • Journal Title

      Genes Cells

      Volume: 18 Pages: 823-37

    • DOI

      10.1111/gtc.12075

  • [Journal Article] A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA2012

    • Author(s)
      Kawakami K, Hayashi A, Nakayama J, & Murakami Y
    • Journal Title

      Genes Dev

      Volume: 26 Pages: 811-1824

    • DOI

      10.1101/gad.190272.112

  • [Journal Article] Intrinsic nucleic acid-binding activity of Chp1 chromodomain is required for heterochromatic gene silencing2012

    • Author(s)
      Ishida M, Shimojo H, Hayashi A, Kawaguchi R, Ohtani Y, Uegaki K, Nishimura Y & Nakayama J
    • Journal Title

      Mol. Cell

      Volume: 47 Pages: 228-41

    • DOI

      10.1016/j.molcel.2012.05.017

  • [Journal Article] Two different RFC proteins, Ctf18 and RFC1 separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV-irradiation2012

    • Author(s)
      Shiomi Y, Ishii T, Hayashi A, Shinmyozu K, Nakayama J, Sugasawa K, & Nishitani H
    • Journal Title

      Mol Cell Biol

      Volume: 32 Pages: 2279-88

    • DOI

      10.1128/MCB.06506-11

  • [Journal Article] Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly2012

    • Author(s)
      Hayashi A, Ishida M, Kawaguchi R, Urano T, Murakami Y, & Nakayama J
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109 Pages: 6159-64

    • DOI

      10.1073/pnas.1116972109

  • [Journal Article] Fub1p, a novel protein isolated by boundary screening, binds the proteasome complex2011

    • Author(s)
      Hatanaka A, Chen B, Sun JQ, Mano Y, Funakoshi M, Kobayashi H, Ju Y, Mizutani T, Shinmyozu K, Nakayama J, Miyamoto K, Uchida H, & Oki M
    • Journal Title

      Genes Genet Syst

      Volume: 86 Pages: 305-314

    • URL

      https://www.jstage.jst.go.jp/article/ggs/86/5/86_5_305/_article

  • [Journal Article] RNA and epigenetic silencing : Insight from fission yeast2011

    • Author(s)
      Goto DB & Nakayama J
    • Journal Title

      Dev Growth Differ

      Volume: 54 Pages: 129-141

    • DOI

      10.1111/j.1440-169X.2011.01310.x

  • [Journal Article] Roles of fission yeast Grc3 in ribosomal RNA processing and heterochromatic gene silencing2011

    • Author(s)
      Kitano E, Hayashi A, Kanai D, Shinmyozu K, & Nakayama J
    • Journal Title

      J. Biol. Chem

      Volume: 286 Pages: 15391-402

    • DOI

      10.1074/jbc.M110.201343

  • [Presentation] Posttranslational modifications of HP1 regulate its function in heterochromatin formation2014

    • Author(s)
      中山潤 一
    • Organizer
      Keystone Symposia "Chromatin mechanisms and cell physiology"
    • Place of Presentation
      Oberstdorf Haus(ドイツ, オーベルストドルフ)
    • Year and Date
      2014-03-25
  • [Presentation] ヘテロクロマチンタンパク質HP1 による高次クロマチン構造形成の分子機構2013

    • Author(s)
      中山潤一
    • Organizer
      平成25年度遺伝研研究会
    • Place of Presentation
      国立遺伝学研究所(静岡県三島市)
    • Year and Date
      2013-10-17
  • [Presentation] ヘテロクロマチンタンパク質HP1 による高次クロマチン構造形成のメカニズム2013

    • Author(s)
      中山潤一
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2013-09-12
  • [Presentation] Roles of chromodomain proteins in higher-order chromatin assembly2013

    • Author(s)
      中山潤一
    • Organizer
      エピジェネティクス国際シンポジウム
    • Place of Presentation
      グランディア芳泉(福井県あわら市)
    • Year and Date
      2013-09-02
  • [Presentation] ヒストン脱メチル化酵素RBP2 と相互作用する因子の探索と転写調節における役割の解明2012

    • Author(s)
      西淵剛平
    • Organizer
      第35回日本分子生物学会年
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      20121211-14
  • [Presentation] 分裂酵母の遺伝子サイレンシングにおけるクロモドメインタンパク質の役割2012

    • Author(s)
      中山潤一
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      20121211-14
  • [Presentation] ヒストン脱メチル化酵素RBP2 と相互作用する因子の探索と転写調節における役割の解明2012

    • Author(s)
      西淵剛平
    • Organizer
      第6回エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Year and Date
      20120514-15
  • [Presentation] 分裂酵母ヘテロクロマチン形成におけるChp1 の役割2012

    • Author(s)
      中山潤一
    • Organizer
      第29回染色体ワークショップ
    • Place of Presentation
      ホテルニュー水戸屋(仙台市)
    • Year and Date
      2012-01-26
  • [Presentation] Roles of chromodomain proteins in higher-order chromatin assembly2011

    • Author(s)
      中山潤一
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2011-12-15
  • [Presentation] 高次クロマチン構造の形成と維持の分子機構2011

    • Author(s)
      中山潤一
    • Organizer
      第62回染色体学会年会
    • Place of Presentation
      神奈川大学(神奈川県平塚市)
    • Year and Date
      2011-11-12
  • [Presentation] Roles of chromodomain proteins in RNAi-directed heterochromatin assembly2011

    • Author(s)
      中山潤一
    • Organizer
      第6回国際分裂酵母学会
    • Place of Presentation
      ボストン(アメリカ)
    • Year and Date
      2011-06-30
  • [Book] エピジェネティクスキーワード事典2013

    • Author(s)
      中山潤一, 他
    • Total Pages
      43-51
    • Publisher
      羊土社
  • [Book] エピジェネティクス2013

    • Author(s)
      中山潤一, 他
    • Total Pages
      49-65
    • Publisher
      化学同人
  • [Book] 染色体と細胞核のダイナミクス2013

    • Author(s)
      中山潤一, 他
    • Total Pages
      79-93
    • Publisher
      化学同人
  • [Book] ヘテロクロマチン構造の形成とサイレンシング2013

    • Author(s)
      中山潤 一
    • Total Pages
      565-70
    • Publisher
      日本生化学会生化学
  • [Book] 実験医学2012年9月号「ヘテロクロマチン構造からみる非コードDNA と染色体」2012

    • Author(s)
      中山潤一
    • Total Pages
      2221-2227
    • Publisher
      羊土社
  • [Book] ヘテロクロマチンタンパク質HP12011

    • Author(s)
      中山潤一
    • Total Pages
      466-467
    • Publisher
      生体の科学
  • [Book] 高次クロマチン構造とノンコーディングRNA2011

    • Author(s)
      中山潤一
    • Total Pages
      699-705
    • Publisher
      秀潤社, 細胞工学
  • [Remarks]

    • URL

      http://www.nsc.nagoya-cu.ac.jp/~jnakayam/

URL: 

Published: 2015-06-25  

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